Chromatography MCQs : Here you will find MCQ Questions related to "Chromatography" in Bioprocess Engineering. These Chromatography MCQ Questions Will help you to improve your Bioprocess Engineering knowledge and will prepare you for various Examinations like Competitive Exams, Placements, Interviews and other Entrance Exmaniations.
Question 1
________________ is a laboratory technique for the separation of a mixture.
A. Centrifugation
B. Adsorption
C. Chromatography
D. Filtration
View Answer
Ans : C
Explanation: Chromatography is a laboratory technique for the separation of a mixture.
Question 2
The mixture is dissolved in a fluid (gas, solvent, water, ...) called the ?
A. fluid phase
B. mobile phase
C. system phase
D. chromatography phase
View Answer
Ans : B
Explanation: The mixture is dissolved in a fluid (gas, solvent, water, ...) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which is fixed a material called the stationary phase.
Question 3
Chromatography was first devised in Russia by the Italian-born scientist Mikhail Tsvet in ?
A. 1895
B. 1890
C. 1905
D. 1900
View Answer
Ans : D
Explanation: Chromatography was first devised in Russia by the Italian-born scientist Mikhail Tsvet in 1900. He developed the technique, he coined chromatography, in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll, carotenes, and xanthophylls.
Question 4
The analyte is the substance to be separated during chromatography
A. TRUE
B. FALSE
C. Can be true or false
D. Can not say
View Answer
Ans : A
Explanation: True, The analyte is the substance to be separated during chromatography
Question 5
A _____________ is the visual output of the chromatograph
A. chromatography
B. aerograph
C. chromatogram
D. All of the above
View Answer
Ans : C
Explanation: A chromatogram is the visual output of the chromatograph. In the case of an optimal separation, different peaks or patterns on the chromatogram correspond to different components of the separated mixture.
Question 6
Which type of mechanism is applicable to chromatography?
A. Absorption and Desorption
B. Adsorption and Desorption
C. Adsorption and Absorption
D. Adsorption and Diffusion
View Answer
Ans : B
Explanation: Adsorption means the attraction to a substance (as opposed to absorption, which means the complete envelopment of something). Desorption is the opposite – when something is not attracted to the substance.
Question 7
Which type of chromatography depends on the principle of size of particles?
A. Affinity chromatography
B. Ion- exchange chromatography
C. Multimodal chromatography
D. Gel- filtration chromatography
View Answer
Ans : D
Explanation: Gel filtration chromatography, a type of size exclusion chromatography, can be used to either fractionate molecules and complexes in a sample into fractions with a particular size range, to remove all molecules larger than a particular size from the sample, or a combination of both operations
Question 8
What useful information can be found from a Van Deemter plot?
A. Optimum column length
B. Optimum column temperature
C. Optimum mobile phase flow rate
D. The selectivity factor
View Answer
Ans : A
Explanation: The Van Deemter equation is an empirical formula describing the relationship between plate height (H, the length needed for one theoretical plate) which is a measure of column efficiency, and linear velocity (µ) .
Question 9
Theoretical plates are used to ___________
A. Determine the thickness of the mobile phase
B. Determine the thickness of the stationary phase
C. Estimate the efficiency of the column
D. Measure the distribution of the analyte between mobile and stationary phases
View Answer
Ans : C
Explanation: The plate model supposes that the chromatographic column contains a large number of separate layers, called theoretical plates. Separate equilibrations of the sample between the stationary and mobile phase occur in these “plates”.
Question 10
The eluate is the mobile phase leaving the column. This is also called effluent.
A. TRUE
B. FALSE
C. Can be true or false
D. Can not say
View Answer
Ans : A
Explanation: True, The eluate is the mobile phase leaving the column. This is also called effluent.