Polymerases MCQs : Here you will find MCQ Questions related to "Polymerases" in Genetic Engineering. These Polymerases MCQ Questions Will help you to improve your Genetic Engineering knowledge and will prepare you for various Examinations like Competitive Exams, Placements, Interviews and other Entrance Exmaniations.
A polymerase is ?
D. All of the above
Explanation: A polymerase is an enzyme that synthesizes long chains of polymers or nucleic acids.
Which of the following are used to assemble DNA and RNA molecules?
A. DNA Polymerase
B. RNA Polymerase
C. Both A and B
D. None of the above
Explanation: DNA polymerase and RNA polymerase are used to assemble DNA and RNA molecules, respectively, by copying a DNA template strand using base-pairing interactions or RNA by half ladder replication.
A polymerase always template dependent?
C. Can be yes or no
D. Can not say
Explanation: A polymerase may be template dependent or template independent.
Which of the following known to have template independent and template dependent activities?
A. Terminal deoxynucleotidyl transferase
C. Both A and B
Explanation: Terminal deoxynucleotidyl transferase also known to have template independent and template dependent activities.
RNA dependent DNA polymerase also known as?
A. DNA polymerases
B. RNA polymerases
D. Reverse transcriptase
Explanation: RNA dependent DNA polymerase known as Reverse transcriptase
Poly-A-polymerase is an example of template independent polymerase.
C. Can be true or false
D. Can not say
Explanation: Poly-A-polymerase is an example of template independent polymerase.
Basic classification of polymerases includes how many types?
Explanation: The types are- DNA dependent DNA polymerase, DNA dependent RNA polymerase, RNA dependent DNA polymerase, template independent polymerase and RNA dependent RNA polymerase. Out of these, RNA dependent RNA polymerases are not focused so much in comparison to other polymerases
Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________
A. end filling and polishing respectively
B. polishing in both the cases
C. polishing and end filling respectively
D. end filling in both the cases
Explanation: The single stranded ends generated due to exonucleolytic activity can either be removed by generating their complementary strand or removing the single stranded overhang. In both cases, it is termed as polishing. Whereas removal of single stranded regions generated because of polymerase activity is termed as end-filling.
Thermostable DNA polymerases are very important in PCR. How are they obtained?
A. They are obtained by heating the bacteria manually over high temperatures
B. They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents
C. They are found everywhere in nature
D. They are obtained by genetically modifying the E. coli bacteria with thermal stability property
Explanation: Thermostable DNA polymerases are found naturally in thermophilic bacteria which can be found growing in oceanic vents. They are having high stability and their DNA polymerases can function effectively even at high temperatures in-vitro.
Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these?
A. They only add a single nucleotide
B. They only add a string of nucleotides and not a single nucleotide
C. Terminal transferase adds a series of nucleotides at the 3’ end
D. Taq polymerase adds a single nucleotide at the 5’ end of the PCR product
Explanation: Template independent polymerases can either add a single nucleotide or a series of nucleotides; it is based on the template. Terminal transferase adds a series of nucleotides at the 3’ end, which generates a single stranded tail. Whereas, Taq polymerase adds a single A base at 3’ end of the PCR product.