DAPZOI
Home Ask MCQ Quiz Contact Us Login




Polymerase Chain Reaction - Molecular Biology MCQ Questions

Polymerase Chain Reaction MCQs : Here you will find MCQ Questions related to "Polymerase chain reaction" in Molecular Biology. These Polymerase chain reaction MCQ Questions Will help you to improve your Molecular Biology knowledge and will prepare you for various Examinations like Competitive Exams, Placements, Interviews and other Entrance Exmaniations.




Question 1

___________________ is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail.

A. DEA
B. URS
C. PCR
D. ORA

View Answer


Question 2

PCR was invented in?

A. 1980
B. 1981
C. 1982
D. 1983

View Answer


Question 3

PCR amplifies a specific region of a _________ strand.

A. RNA
B. DNA
C. Both A and B
D. None of the above

View Answer


Question 4

PCR consists of a series of 20–40 repeated temperature changes, called thermal cycles.

A. TRUE
B. FALSE
C. Can be true or false
D. Can not say

View Answer


Question 5

Which of the following is the final step that cools the reaction chamber to 4–15 °C (39–59 °F) for an indefinite time.

A. Extension
B. Final elongation
C. Final hold
D. elongation

View Answer


Question 6

Initialization: This step is only required for DNA polymerases that require heat activation by hot-start PCR.

A. TRUE
B. FALSE
C. Can be true or false
D. Can not say

View Answer


Question 7

Which of the following is a mismatch?

A. Polymerase – Taq polymerase
B. Template – double stranded DNA
C. Primer – oligonucleotide
D. Synthesis – 5’ to 3’ direction

View Answer


Question 8

Primer used for the process of polymerase chain reaction are ______________

A. Single stranded DNA oligonucleotide
B. Double stranded DNA oligonucleotide
C. Single stranded RNA oligonucleotide
D. Double stranded RNA oligonucleotide

View Answer


Question 9

How many DNA duplex is obtained from one DNA duplex after 4 cycles of PCR?

A. 2
B. 4
C. 8
D. 16

View Answer


Question 10

With respect to target DNA used in inverted PCR which of the following is not true?

A. Restricted segment
B. Blunt ended segment
C. Intact known segment
D. Flanked unknown segment flanked on either side

View Answer