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Animal Cell Culture and Regulation MCQ Questions & Answers

Animal Cell Culture and Regulation MCQs : This section focuses on the "Animal Cell Culture and Regulation". These Multiple Choice Questions (MCQs) should be practiced to improve the Animal Cell Culture and Regulation skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

Higher dissolved oxygen concentration in the culture media are toxic and lead to

A. DNA degradation
B. lipid peroxidation
C. metabolism of nutrients in culture media at a rate greater than that required for consumption
D. all of the above

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Question 2

Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

A. established cell lines
B. primary cell lines
C. secondary cell lines
D. propagated cell lines

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Question 3

When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

A. incomplete glutamine oxidation
B. increase in specific lactate production from glucose
C. Both A and B
D. accumulation of ammonia

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Question 4

According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

A. 6 amino acids
B. 8 amino acids
C. 10 amino acids
D. 12 amino acids

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Question 5

The technique of organ culture may be divided on the basis of employing

A. solid medium
B. liquid medium
C. both (a) and (b)
D. semi-solid medium

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Question 6

pH of culture medium is initially controlled by

A. presence of CO2
B. presence of bicarbonate buffer
C. addition of bases
D. none of these

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Question 7

What are the main constituents of culture for animal cell growth?

A. Glucose and Glutamine
B. Growth factors
C. Cytokines
D. All of these

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Question 8

What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

A. They act as growth promoters
B. They act as growth inhibitors
C. Have no effect on cells
D. Lactate helps in the growth while ammonium inhibits the growth

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Question 9

At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

A. oxidative pathway
B. anaerobic pathway
C. both (a) and (b)
D. none of these

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Question 10

The human fibroplast is a classical example of

A. stable primary cell lines
B. established cell lines
C. cell transformations
D. None of the above

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Question 11

Disaggregating of cells can be achieved by

A. physical disruption
B. enzymatic digestion
C. treating with chelating agents
D. All of the above

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Question 12

Excess CO2 suppress cell growth and productivity by

A. inhibiting respiration
B. altering intracellular pH by diffusing across cell membrane
C. both (a) and (b)
D. altering pH of the medium

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Question 13

Which of the followings are the metabolic products of glucose and glutamine?

A. CO2 and NH3
B. CO2 and lactate
C. Lactate and ammonium
D. Lactate only

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Question 14

Range of optimum glucose concentration present in the culture media is

A. 5.5 - 55 mmol/litre
B. 55 - 75 mmol/litre
C. 75-105 mmol/litre
D. 105-150 mmol/litre

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Question 15

Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

A. 0.05-5 nmol of O2/cell/h
B. 5 - 10 nmol of O2 /cell/h
C. 0.05-5 pmol of O2/cell/h
D. 5 - 10 pmol of O2/cell/h

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Question 16

Which of the following is not a source of energy in active muscle cells?

A. Creatine phosphate
B. ATP
C. Lactic acid
D. Glucose

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Question 17

Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

A. Down's syndrome
B. Turner's syndrome
C. Klinefelter's syndrome
D. All of the above

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Question 18

How does CO2 help in the cell metabolism during cell culture?

A. It participates in the de novo synthesis of purines and pyrimidines
B. Helps in the cells respiration
C. For monitoring pH of the culture
D. All of the above

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Question 19

What is the concentration of CO2 required for culturing animal cells?

A. 2-5%
B. 1-10%
C. 10-15%
D. 15-20%

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Question 20

Which of the following is the technique used for the embryo culture?

A. Organ cultures on plasma clots
B. Organ cultures on agar
C. Whole embryo cultures
D. All of these

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Question 21

Excess CO2 suppress cell growth and productivity by

A. inhibiting respiration
B. altering intracellular pH by diffusing across cell membrane
C. Both A and B
D. altering pH of the medium

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Question 22

An established cell line can be called where it has been subcultured at least

A. 70 times at an interval of 3 days between subcultures
B. 40 times at an interval of 3 days between subcultures
C. 70 times at an interval of 1 day between subcultures
D. 50 times at an interval of 3 days between subcultures

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Question 23

The human fibroplast is a classical example of

A. stable primary cell lines
B. established cell lines
C. cell transformations
D. none of these

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Question 24

Which of the following is correct?

A. ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
B. ECL and PCL can be established in suspension cultures
C. PCL can be established in suspension cultures
D. None of the above

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Question 25

The major problem associated with the isolation of free cells and cell aggregates from organs is that of

A. releasing the cells from their supporting matrix
B. inhibiting the cells from their supporting matrix
C. disintegrating the cells from their supporting matrix
D. none of the above

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Question 26

When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

A. incomplete glutamine oxidation
B. increase in specific lactate production from glucose
C. both (a) and (b)
D. accumulation of ammonia

View Answer

Question 27

Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

A. Down's syndrome
B. Turner's syndrome
C. Klinefelter's syndrome
D. all of these

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Question 28

Toxicity due to accumulation of ammonia can be overcome

A. by substituting glutamine by glutamate
B. by controlled addition of glutamine at low level
C. by removal of ammonia or ammonium from culture medium
D. All of the above

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Question 29

Accumulation of lactate leads to

A. increase in pH
B. no change in pH
C. reduction in the pH of culture hence loss of cell viability
D. no loss of cell viability

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Question 30

Cells which have undergone transformation frequently become

A. anchorage independent
B. anchorage dependent
C. stable
D. unstable

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Question 31

Range of optimum glutamine concentration present in the culture media is

A. 1-2 mmol/litre
B. 2-7 mmol/litre
C. 7-15 mmol/litre
D. 15 - 20 mmol/litre

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Question 32

pH of culture medium is initially controlled by

A. presence of CO2
B. presence of bicarbonate buffer
C. addition of bases
D. None of the above

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Question 33

For culturing, plasma from the adult chicken is preferred to mammalian plasma because

A. it forms a clear and solid coagulum even after dilution
B. it is too opaque
C. it doesn't produce solid clots
D. it forms a semi solid coagulum

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Question 34

Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

A. 150-300
B. 280-360
C. 300-325
D. 360-400

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Question 35

In animal cell culture, particularly mammalian cell culture, transformation means

A. uptake of new genetic material
B. phenotypic modifications of cells in culture
C. both (a) and (b)
D. release of genetic information

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Question 36

Which of the following is not the explantation technique?

A. Slide culture
B. Carrel flask culture
C. Roller test tube culture
D. Adherent primary culture

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Question 37

Which of the following is incorrect?

A. Established cell lines (ECL) have short doubling time
B. ECL are invariably aneuploid
C. ECL grow in higher density
D. ECL do not show much evidence of spatial orientation

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Question 38

During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

A. Ethyl alcohol is being produced in excess
B. The cells have too much oxygen
C. Glycolysis is being inhibited
D. The cells do not have enough oxygen

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Question 39

What are the main constituents of culture for animal cell growth?

A. Glucose and Glutamine
B. Growth factors
C. Cytokines
D. All of the above

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Question 40

To prevent the accumulation of lactate

A. low glutamine concentration is required
B. high glutamine concentration is required
C. low glucose concentration is required
D. high glucose concentration is required

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