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Bacteriophages MCQ Questions & Answers

Bacteriophages MCQs : This section focuses on the "Bacteriophages". These Multiple Choice Questions (MCQs) should be practiced to improve the Bacteriophages skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

T-even phage binding to E. coli probably involves

A. electrostatic interaction
B. hydrophobic interaction
C. covalent bonds
D. All of the above

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Question 2

Which of the following is a positive regulatory gene on the lambda phage genome?

A. N
B. O
C. P
D. R

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Question 3

Which of the following properties is due to lysogenic conversion by phages?

A. Toxin production in Corynebacterium diphtheriae
B. Toxin production in Clostridium botulinum types C and D
C. Antigenic variation in Salmonella anatum
D. All of the above

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Question 4

Area of lysis on a bacterial lawn culture produced by a phage is known as

A. pock
B. plaque
C. pox
D. all of these

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Question 5

The process by which phage reproduction is initiated in lysogenized culture is called

A. infection
B. integration
C. repression
D. induction

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Question 6

How are genes arranged in a lysogenic phage (for example a lambda phage)?

A. Alternatively
B. In clusters
C. On opposite ends
D. Similar genes at distant positions

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Question 7

What is the function of a tail of the head-and-tail lambda phage?

A. Stabilization of phage DNA
B. Attachment of phage
C. Release of replicative enzymes
D. Helps in motility

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Question 8

Which capsid symmetry is exhibited by most of the phages?

A. Helical
B. Icosahedral
C. Complex
D. None of these

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Question 9

Area of lysis on a bacterial lawn culture produced by a phage is known as

A. pock
B. plaque
C. pox
D. All of the above

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Question 10

T-even phage binding to E. coli probably involves

A. electrostatic interaction
B. hydrophobic interaction
C. covalent bonds
D. all of these

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Question 11

In viruses with envelopes

A. the envelope and the embedded proteins are encoded by the viral nucleic acid
B. the envelope is derived from the host but it contains embedded proteins coded by the viral nucleic acid
C. the envelope is coded by the viral nucleic acids, but the proteins come from the host's membrane proteins
D. the envelope and its imbedded proteins are derived from the host's membranes

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Question 12

By which mechanism does the replication of new lambda DNA molecules are produced in the host bacterium?

A. Rolling Circle Mechanism
B. DNA Polymerase Binding
C. The same mechanism as that of host
D. Replication fork propagation

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Question 13

The procapsid is assembled with the aid of __________ proteins.

A. ladder
B. framing
C. scaffolding
D. form

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Question 14

How does an injection of a filamentous phage (example- M13) DNA into the host bacterium occur?

A. Via pilus
B. Surface attachment
C. Transformation
D. Transfection

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Question 15

Replicative form (RF) refers to what part of the M13 bacteriophage?

A. M13 genome
B. Restriction sites
C. Endonuclease activity
D. Replication phase

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Question 16

One of the first enzymes synthesized by many bacteriophage is __________ , an RNA-dependent RNA polymerase.

A. RNA transcriptase
B. RNA polymerase
C. RNA ligase
D. RNA replicase

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Question 17

__________ is specifically able to have a long-term relationship with the host known as lysogeny.

A. Temperate virus
B. Adsorbed virus
C. RNA phage
D. DNA phage

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Question 18

M13 filamentous phage DNA molecule is smaller than the head-and-tail lambda phage.

A. True
B. False

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Question 19

The filamentous bacteriopohage infect male E. coli cells by attaching to

A. lipopolysaccharide
B. the cell wall
C. the tip of the pilus
D. the cell membrane

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Question 20

Which of the following bacteria can be typed by phage typing method?

A. Staphylococcus aureus
B. Salmonella typhi
C. Vibrio cholerae
D. All of the above

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Question 21

Phage display technique makes use of which of the following vectors?

A. M13
B. Lambda
C. 2 micron circle
D. BAC

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Question 22

Contractile sheath of the tail is present in which of the following phages?

A. T3
B. T2
C. P22
D. All of the above

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Question 23

Which of the following bacteria can be typed by phage typing method?

A. Staphylococcus aureus
B. Salmonella typhi
C. Vibrio cholerae
D. All of these

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Question 24

Which infection cycle is characterized by retention of the phage DNA molecule in the host bacterium for many thousands of cell division?

A. Lysogenic cycle
B. Lytic cycle
C. Integrative Phase
D. Protein synthesis

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Question 25

Which of these is not a feature associated with M13 phage?

A. Doesn’t need genes for insertion into host genome
B. Capsid constructed of multiple copies of 3 genes
C. Simpler infection cycle than lambda
D. Capsid constructed of 15 different proteins

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Question 26

Contractile sheath of the tail is present in which of the following phages?

A. T3
B. T2
C. P22
D. All of these

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Question 27

The replication of phage DNA molecule is associated with which step in the infection cycle of a bacteriophage?

A. First step
B. Second step
C. Third step
D. Preparation stage

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Question 28

__________ protein keeps the prophage dormant and prevents virus reproduction.

A. Operator
B. Promotor
C. Repressor
D. Enhancer

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Question 29

Circularization of the injected linear phage DNA molecule is facilitated by which of the following?

A. Polyhedral head
B. Cos sites
C. Phage tail
D. Capsid proteins

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Question 30

Which of the following is/are synthesized from late mRNA?

A. Phage structural proteins
B. Proteins that help with phage assembly without becoming part of the virion structure
C. Proteins involved in cell lysis and phage release
D. All of the above

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Question 31

A bacterial defense mechanism against bacteriophage is called

A. concatamerization
B. polymerization
C. restriction
D. lysogeny

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