Bacteriophages MCQs : This section focuses on the "Bacteriophages". These Multiple Choice Questions (MCQs) should be practiced to improve the Bacteriophages skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Question 1
The process by which phage reproduction is initiated in lysogenized culture is called
A. infection
B. integration
C. repression
D. induction
Question 2
Area of lysis on a bacterial lawn culture produced by a phage is known as
A. pock
B. plaque
C. pox
D. All of the above
Question 3
The procapsid is assembled with the aid of __________ proteins.
A. ladder
B. framing
C. scaffolding
D. form
Question 4
Which of the following is/are synthesized from late mRNA?
A. Phage structural proteins
B. Proteins that help with phage assembly without becoming part of the virion structure
C. Proteins involved in cell lysis and phage release
D. All of the above
Question 5
Which capsid symmetry is exhibited by most of the phages?
A. Helical
B. Icosahedral
C. Complex
D. None of these
Question 6
Contractile sheath of the tail is present in which of the following phages?
A. T3
B. T2
C. P22
D. All of the above
Question 7
One of the first enzymes synthesized by many bacteriophage is __________ , an RNA-dependent RNA polymerase.
A. RNA transcriptase
B. RNA polymerase
C. RNA ligase
D. RNA replicase
Question 8
In viruses with envelopes
A. the envelope and the embedded proteins are encoded by the viral nucleic acid
B. the envelope is derived from the host but it contains embedded proteins coded by the viral nucleic acid
C. the envelope is coded by the viral nucleic acids, but the proteins come from the host's membrane proteins
D. the envelope and its imbedded proteins are derived from the host's membranes
Question 9
Which of the following bacteria can be typed by phage typing method?
A. Staphylococcus aureus
B. Salmonella typhi
C. Vibrio cholerae
D. All of the above
Question 10
__________ protein keeps the prophage dormant and prevents virus reproduction.
A. Operator
B. Promotor
C. Repressor
D. Enhancer
Question 11
__________ is specifically able to have a long-term relationship with the host known as lysogeny.
A. Temperate virus
B. Adsorbed virus
C. RNA phage
D. DNA phage
Question 12
Which of the following properties is due to lysogenic conversion by phages?
A. Toxin production in Corynebacterium diphtheriae
B. Toxin production in Clostridium botulinum types C and D
C. Antigenic variation in Salmonella anatum
D. All of the above
Question 13
T-even phage binding to E. coli probably involves
A. electrostatic interaction
B. hydrophobic interaction
C. covalent bonds
D. All of the above
Question 14
The filamentous bacteriopohage infect male E. coli cells by attaching to
A. lipopolysaccharide
B. the cell wall
C. the tip of the pilus
D. the cell membrane
Question 15
A bacterial defense mechanism against bacteriophage is called
A. concatamerization
B. polymerization
C. restriction
D. lysogeny
Question 16
Area of lysis on a bacterial lawn culture produced by a phage is known as
A. pock
B. plaque
C. pox
D. all of these
Question 17
Contractile sheath of the tail is present in which of the following phages?
A. T3
B. T2
C. P22
D. All of these
Question 18
T-even phage binding to E. coli probably involves
A. electrostatic interaction
B. hydrophobic interaction
C. covalent bonds
D. all of these
Question 19
Which of the following bacteria can be typed by phage typing method?
A. Staphylococcus aureus
B. Salmonella typhi
C. Vibrio cholerae
D. All of these
Question 20
By which mechanism does the replication of new lambda DNA molecules are produced in the host bacterium?
A. Rolling Circle Mechanism
B. DNA Polymerase Binding
C. The same mechanism as that of host
D. Replication fork propagation
Question 21
Circularization of the injected linear phage DNA molecule is facilitated by which of the following?
A. Polyhedral head
B. Cos sites
C. Phage tail
D. Capsid proteins
Question 22
How are genes arranged in a lysogenic phage (for example a lambda phage)?
A. Alternatively
B. In clusters
C. On opposite ends
D. Similar genes at distant positions
Question 23
How does an injection of a filamentous phage (example- M13) DNA into the host bacterium occur?
A. Via pilus
B. Surface attachment
C. Transformation
D. Transfection
Question 24
M13 filamentous phage DNA molecule is smaller than the head-and-tail lambda phage.
A. True
B. False
Question 25
Replicative form (RF) refers to what part of the M13 bacteriophage?
A. M13 genome
B. Restriction sites
C. Endonuclease activity
D. Replication phase
Question 26
Phage display technique makes use of which of the following vectors?
A. M13
B. Lambda
C. 2 micron circle
D. BAC
Question 27
The replication of phage DNA molecule is associated with which step in the infection cycle of a bacteriophage?
A. First step
B. Second step
C. Third step
D. Preparation stage
Question 28
What is the function of a tail of the head-and-tail lambda phage?
A. Stabilization of phage DNA
B. Attachment of phage
C. Release of replicative enzymes
D. Helps in motility
Question 29
Which infection cycle is characterized by retention of the phage DNA molecule in the host bacterium for many thousands of cell division?
A. Lysogenic cycle
B. Lytic cycle
C. Integrative Phase
D. Protein synthesis
Question 30
Which of the following is a positive regulatory gene on the lambda phage genome?
A. N
B. O
C. P
D. R
Question 31
Which of these is not a feature associated with M13 phage?
A. Doesn’t need genes for insertion into host genome
B. Capsid constructed of multiple copies of 3 genes
C. Simpler infection cycle than lambda
D. Capsid constructed of 15 different proteins