Chromatography MCQs : This section focuses on the "Chromatography". These Multiple Choice Questions (MCQs) should be practiced to improve the Chromatography skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Question 1
Which of the following dye is widely used in dye-ligand chromatography?
A. Methyl blue
B. Cibracron blue
C. Methyl orange
D. Malachite green
Question 2
In reverse phase chromatography, the stationary phase is made
A. non-polar
B. polar
C. either non-polar or polar
D. none of these
Question 3
Which of the following is not a stage of product recovery?
A. Removal of solids
B. Isolation of organism
C. Purification and concentration
D. Cell disruption
Question 4
Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography.
A. TRUE
B. FALSE
Question 5
Which of the following is not a gel filtration chromatography?
A. Molecular sieve
B. Gel permeation
C. Size exclusion
D. Gel residue
Question 6
The visual output of chromatography is called?
A. Chromatograph
B. Chromatogram
C. Electropherogram
D. Autoradiogram
Question 7
In which of the following type of chromatography the capillary action mechanism is present?
A. Liquid chromatography
B. Gas chromatography
C. Thin- Layer chromatography
D. Paper chromatography
Question 8
Which type of mechanism is applicable to chromatography?
A. Absorption and Desorption
B. Adsorption and Absorption
C. Adsorption and Desorption
D. Adsorption and Diffusion
Question 9
Which of the following is not a criterion for the choice of the recovery process?
A. Location of the product
B. Price of the product
C. Use of the product
D. Source of organism
Question 10
Chromatography was first devised in Russia by the Italian-born scientist Mikhail Tsvet in 1900.
A. TRUE
B. FALSE
Question 11
What does the retention factor, k’, describe?
A. The velocity from the stationary phase
B. The velocity of the mobile phase
C. The distribution of an analyte between the stationary and the mobile phase
D. The migration rate of an analyte through a column
Question 12
Which type of chromatography depends on the principle of size of particles?
A. Affinity chromatography
B. Gel- filtration chromatography
C. Ion- exchange chromatography
D. Multimodal chromatography
Question 13
The charged molecules can be separated by __________
A. Column chromatography
B. Ion exchange chromatography
C. Thin layer chromatography
D. Affinity chromatography
Question 14
Which of the following is used to separate molecules based on affinity?
A. Column chromatography
B. Ion exchange chromatography
C. Thin layer chromatography
D. Affinity chromatography
Question 15
Thin layer chromatography is
A. partition chromatography
B. electrical mobility of ionic species
C. adsorption chromatography
D. none of the above
Question 16
Chromatography is used to separate ____________
A. Complex mixture compounds
B. Simple mixtures
C. Viscous mixtures
D. Halogens
Question 17
The Rf value is the ratio of distance travelled by solvent to the distance travelled by the solute.
A. True
B. False
Question 18
The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)
A. V = V0 + kDVi
B. V = V0/Vi
C. V = V0 - kDVi
D. V/V0 = kDVi
Question 19
Ion exchange chromatography is based on the
A. electrostatic attraction
B. electrical mobility of ionic species
C. adsorption chromatography
D. partition chromatography
Question 20
In gas chromatography, the basis for separation of the components of the volatile material is the difference in
A. partition coefficients
B. conductivity
C. molecular weight
D. molarity
Question 21
Who employed the term ‘Chromatography’?
A. Tsvet
B. Archer
C. Richard
D. Erika
Question 22
Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis.
A. TRUE
B. FALSE
Question 23
A combination of paper chromatography and electrophoresis involves
A. partition chromatography
B. electrical mobility of the ionic species
C. both (a) and (b)
D. none of these
Question 24
Which of the following condition is of reverse phase chromatography?
A. The mobile phase is non-polar and stationary phase is polar
B. The mobile phase is polar and stationary phase is non-polar
C. Both the mobile phase and stationary phase are organic
D. Both the mobile phase and stationary phase are inorganic
Question 25
Which of the following is used in the precipitation of dextrans?
A. Methanol
B. PEG
C. Ammonium sulphate
D. Sodium sulphate
Question 26
Chromatography technique developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s, for which they won the 1952 Nobel Prize in Chemistry.
A. TRUE
B. FALSE
Question 27
According to the small size of the particle, which type of chromatographic separation is applicable?
A. High- performance liquid chromatography (HPLC)
B. Fast protein liquid chromatography (FPLC)
C. Gel chromatography
D. Paper chromatography
Question 28
Thin Layer Chromatography is similar to Paper Chromatography.
A. True
B. False
Question 29
The HPLC uses the application of ___________
A. High temperature
B. Low temperature
C. High pressure
D. Low pressure
Question 30
Which of the following is used to pack columns in adsorption chromatography?
A. Carbon
B. Silica gel
C. Potassium hydroxide
D. Aluminium oxide
Question 31
Immunoaffinity chromatography is used for the purification of ____________
A. Lipoproteins
B. Interferons
C. Antibodies
D. Carbohydrates
Question 32
Which of the following stationary phase is not used in gel filtration chromatography?
A. Sephadex
B. Sephacryl
C. Bio-Gel
D. Resin beads