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Chromatography MCQ Questions & Answers

Chromatography MCQs : This section focuses on the "Chromatography". These Multiple Choice Questions (MCQs) should be practiced to improve the Chromatography skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

What does the retention factor, k’, describe?

A. The velocity from the stationary phase
B. The velocity of the mobile phase
C. The distribution of an analyte between the stationary and the mobile phase
D. The migration rate of an analyte through a column

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Question 2

Which of the following is used in the precipitation of dextrans?

A. Methanol
B. PEG
C. Ammonium sulphate
D. Sodium sulphate

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Question 3

The Rf value is the ratio of distance travelled by solvent to the distance travelled by the solute.

A. True
B. False

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Question 4

The charged molecules can be separated by __________

A. Column chromatography
B. Ion exchange chromatography
C. Thin layer chromatography
D. Affinity chromatography

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Question 5

Which of the following is not a gel filtration chromatography?

A. Molecular sieve
B. Gel permeation
C. Size exclusion
D. Gel residue

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Question 6

Ion exchange chromatography is based on the

A. electrostatic attraction
B. electrical mobility of ionic species
C. adsorption chromatography
D. partition chromatography

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Question 7

Thin layer chromatography is

A. partition chromatography
B. electrical mobility of ionic species
C. adsorption chromatography
D. none of the above

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Question 8

Immunoaffinity chromatography is used for the purification of ____________

A. Lipoproteins
B. Interferons
C. Antibodies
D. Carbohydrates

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Question 9

Which of the following dye is widely used in dye-ligand chromatography?

A. Methyl blue
B. Cibracron blue
C. Methyl orange
D. Malachite green

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Question 10

Which of the following stationary phase is not used in gel filtration chromatography?

A. Sephadex
B. Sephacryl
C. Bio-Gel
D. Resin beads

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Question 11

Which type of chromatography depends on the principle of size of particles?

A. Affinity chromatography
B. Gel- filtration chromatography
C. Ion- exchange chromatography
D. Multimodal chromatography

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Question 12

Which of the following condition is of reverse phase chromatography?

A. The mobile phase is non-polar and stationary phase is polar
B. The mobile phase is polar and stationary phase is non-polar
C. Both the mobile phase and stationary phase are organic
D. Both the mobile phase and stationary phase are inorganic

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Question 13

The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)

A. V = V0 + kDVi
B. V = V0/Vi
C. V = V0 - kDVi
D. V/V0 = kDVi

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Question 14

Chromatography was first devised in Russia by the Italian-born scientist Mikhail Tsvet in 1900.

A. TRUE
B. FALSE

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Question 15

Which of the following is not a criterion for the choice of the recovery process?

A. Location of the product
B. Price of the product
C. Use of the product
D. Source of organism

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Question 16

A combination of paper chromatography and electrophoresis involves

A. partition chromatography
B. electrical mobility of the ionic species
C. both (a) and (b)
D. none of these

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Question 17

Which of the following is used to separate molecules based on affinity?

A. Column chromatography
B. Ion exchange chromatography
C. Thin layer chromatography
D. Affinity chromatography

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Question 18

Which of the following is not a stage of product recovery?

A. Removal of solids
B. Isolation of organism
C. Purification and concentration
D. Cell disruption

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Question 19

Chromatography is used to separate ____________

A. Complex mixture compounds
B. Simple mixtures
C. Viscous mixtures
D. Halogens

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Question 20

Thin Layer Chromatography is similar to Paper Chromatography.

A. True
B. False

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Question 21

In gas chromatography, the basis for separation of the components of the volatile material is the difference in

A. partition coefficients
B. conductivity
C. molecular weight
D. molarity

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Question 22

Who employed the term ‘Chromatography’?

A. Tsvet
B. Archer
C. Richard
D. Erika

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Question 23

Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis.

A. TRUE
B. FALSE

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Question 24

The HPLC uses the application of ___________

A. High temperature
B. Low temperature
C. High pressure
D. Low pressure

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Question 25

In which of the following type of chromatography the capillary action mechanism is present?

A. Liquid chromatography
B. Gas chromatography
C. Thin- Layer chromatography
D. Paper chromatography

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Question 26

Which of the following is used to pack columns in adsorption chromatography?

A. Carbon
B. Silica gel
C. Potassium hydroxide
D. Aluminium oxide

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Question 27

Chromatography technique developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s, for which they won the 1952 Nobel Prize in Chemistry.

A. TRUE
B. FALSE

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Question 28

The visual output of chromatography is called?

A. Chromatograph
B. Chromatogram
C. Electropherogram
D. Autoradiogram

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Question 29

In reverse phase chromatography, the stationary phase is made

A. non-polar
B. polar
C. either non-polar or polar
D. none of these

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Question 30

Which type of mechanism is applicable to chromatography?

A. Absorption and Desorption
B. Adsorption and Absorption
C. Adsorption and Desorption
D. Adsorption and Diffusion

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Question 31

Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography.

A. TRUE
B. FALSE

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Question 32

According to the small size of the particle, which type of chromatographic separation is applicable?

A. High- performance liquid chromatography (HPLC)
B. Fast protein liquid chromatography (FPLC)
C. Gel chromatography
D. Paper chromatography

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