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Chromatography MCQ Questions & Answers

Chromatography MCQs : This section focuses on the "Chromatography". These Multiple Choice Questions (MCQs) should be practiced to improve the Chromatography skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

Thin layer chromatography is

A. partition chromatography
B. electrical mobility of ionic species
C. adsorption chromatography
D. none of the above

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Question 2

Ion exchange chromatography is based on the

A. electrostatic attraction
B. electrical mobility of ionic species
C. adsorption chromatography
D. partition chromatography

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Question 3

A combination of paper chromatography and electrophoresis involves

A. partition chromatography
B. electrical mobility of the ionic species
C. both (a) and (b)
D. none of these

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Question 4

In reverse phase chromatography, the stationary phase is made

A. non-polar
B. polar
C. either non-polar or polar
D. none of these

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Question 5

In gas chromatography, the basis for separation of the components of the volatile material is the difference in

A. partition coefficients
B. conductivity
C. molecular weight
D. molarity

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Question 6

The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)

A. V = V0 + kDVi
B. V = V0/Vi
C. V = V0 - kDVi
D. V/V0 = kDVi

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Question 7

Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis.

A. TRUE
B. FALSE

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Question 8

Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography.

A. TRUE
B. FALSE

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Question 9

Chromatography was first devised in Russia by the Italian-born scientist Mikhail Tsvet in 1900.

A. TRUE
B. FALSE

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Question 10

Chromatography technique developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s, for which they won the 1952 Nobel Prize in Chemistry.

A. TRUE
B. FALSE

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Question 11

Chromatography is used to separate ____________

A. Complex mixture compounds
B. Simple mixtures
C. Viscous mixtures
D. Halogens

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Question 12

Immunoaffinity chromatography is used for the purification of ____________

A. Lipoproteins
B. Interferons
C. Antibodies
D. Carbohydrates

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Question 13

The charged molecules can be separated by __________

A. Column chromatography
B. Ion exchange chromatography
C. Thin layer chromatography
D. Affinity chromatography

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Question 14

The HPLC uses the application of ___________

A. High temperature
B. Low temperature
C. High pressure
D. Low pressure

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Question 15

The Rf value is the ratio of distance travelled by solvent to the distance travelled by the solute.

A. True
B. False

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Question 16

Thin Layer Chromatography is similar to Paper Chromatography.

A. True
B. False

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Question 17

Which of the following dye is widely used in dye-ligand chromatography?

A. Methyl blue
B. Cibracron blue
C. Methyl orange
D. Malachite green

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Question 18

Which of the following is not a criterion for the choice of the recovery process?

A. Location of the product
B. Price of the product
C. Use of the product
D. Source of organism

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Question 19

Which of the following is not a gel filtration chromatography?

A. Molecular sieve
B. Gel permeation
C. Size exclusion
D. Gel residue

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Question 20

Which of the following is not a stage of product recovery?

A. Removal of solids
B. Isolation of organism
C. Purification and concentration
D. Cell disruption

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Question 21

Which of the following is used in the precipitation of dextrans?

A. Methanol
B. PEG
C. Ammonium sulphate
D. Sodium sulphate

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Question 22

Which of the following is used to pack columns in adsorption chromatography?

A. Carbon
B. Silica gel
C. Potassium hydroxide
D. Aluminium oxide

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Question 23

Which of the following is used to separate molecules based on affinity?

A. Column chromatography
B. Ion exchange chromatography
C. Thin layer chromatography
D. Affinity chromatography

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Question 24

Which of the following stationary phase is not used in gel filtration chromatography?

A. Sephadex
B. Sephacryl
C. Bio-Gel
D. Resin beads

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Question 25

Who employed the term ‘Chromatography’?

A. Tsvet
B. Archer
C. Richard
D. Erika

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Question 26

According to the small size of the particle, which type of chromatographic separation is applicable?

A. High- performance liquid chromatography (HPLC)
B. Fast protein liquid chromatography (FPLC)
C. Gel chromatography
D. Paper chromatography

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Question 27

In which of the following type of chromatography the capillary action mechanism is present?

A. Liquid chromatography
B. Gas chromatography
C. Thin- Layer chromatography
D. Paper chromatography

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Question 28

The visual output of chromatography is called?

A. Chromatograph
B. Chromatogram
C. Electropherogram
D. Autoradiogram

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Question 29

What does the retention factor, k’, describe?

A. The velocity from the stationary phase
B. The velocity of the mobile phase
C. The distribution of an analyte between the stationary and the mobile phase
D. The migration rate of an analyte through a column

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Question 30

Which of the following condition is of reverse phase chromatography?

A. The mobile phase is non-polar and stationary phase is polar
B. The mobile phase is polar and stationary phase is non-polar
C. Both the mobile phase and stationary phase are organic
D. Both the mobile phase and stationary phase are inorganic

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Question 31

Which type of chromatography depends on the principle of size of particles?

A. Affinity chromatography
B. Gel- filtration chromatography
C. Ion- exchange chromatography
D. Multimodal chromatography

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Question 32

Which type of mechanism is applicable to chromatography?

A. Absorption and Desorption
B. Adsorption and Absorption
C. Adsorption and Desorption
D. Adsorption and Diffusion

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