Cloning Vectors MCQs : This section focuses on the "Cloning Vectors". These Multiple Choice Questions (MCQs) should be practiced to improve the Cloning Vectors skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Question 1
Difference between λ gt 10 and λ gt 11 vectors is that
A. λ gt 11 is an expression vector
B. λ gt 10 is an expression vector
C. λ gt 10 is a replacement vector
D. λ gt 11 is a replacement vector
Question 2
Stuffer is
A. the right arm of the vector DNA
B. the left arm of the vector DNA
C. central fragment of the vector DNA
D. None of these
Question 3
Insertion of recombinant DNA within the gene encoding for β–galactosidase leads to ________
A. amplification
B. transformation
C. insertional inactivation
D. cloning
Question 4
A plasmid can be considered as a suitable cloning vector if
A. it can be readily isolated from the cells
B. it possesses a single restriction site for one or more restriction enzymes
C. insertion of foreign DNA does not alter its replication properties
D. All of the above
Question 5
Charon vectors are different from EMBL vectors because
A. they have more extensive range of restriction targets with in their polylinkers.
B. physical separation of lambda arm from central fragment is required
C. Both A and B
D. physical separation of lambda arm from central fragment is not required
Question 6
Select the wrong statement about plasmids?
A. It is extrachromosomal
B. It is double stranded
C. Its replication depends upon host cell
D. It is closed and circular DNA
Question 7
Maximum size of foreign DNA that can be inserted into a replacement vector is
A. 25-30 kb
B. 18-20 kb
C. 20-25 kb
D. 40-50 kb
Question 8
Plasmids which are maintained as limited number of copies per cell are known as
A. stringent plasmids
B. relaxed plasmids
C. cryptic plasmids
D. all of these
Question 9
Cryptic plasmids
A. do not exhibit any phenotypic trait
B. exhibit many phenotypic traits
C. exhibit one phenotypic traits
D. exhibit antibiotic resistance
Question 10
________ is an example of antibiotic.
A. Virus
B. Restriction endonuclease
C. RNA
D. Kanamycin
Question 11
Size of the DNA that can be packaged into a λ phage is
A. 50 kb
B. 35-53 kb
C. 40-50 kb
D. any size
Question 12
Plasmids and ________ have the ability to replicate within bacterial cells independent of the control of chromosomal DNA.
A. bacteriophages
B. fragments
C. bacteria
D. clones
Question 13
The sequence of DNA from where replication starts is called _______
A. selectable marker
B. origin of replication
C. ter sequence
D. genetic sequence
Question 14
Cosmid vectors are used for
A. cloning small fragments of DNA
B. cloning large fragments of DNA
C. cloning prokaryotic DNA only
D. cloning eukaryotic DNA only
Question 15
Cos site of the cosmids
A. consists of 12 bases
B. helps whole genome in circularization and ligation
C. Both A and B
D. contains cleavage site
Question 16
Charon 34 and Charon 35 are the examples of
A. plasmid vector
B. cosmid vector
C. phage vector
D. phagemid vector
Question 17
Cosmids lack
A. genes coding for viral proteins
B. origin of replication
C. marker genes coding for replication
D. cleavage site for the insertion of foreign DNA
Question 18
Single stranded vectors are useful
A. for sequencing of cloned DNA
B. for oligonucleotide directed mutagenesis
C. for probe preparation
D. All of the above
Question 19
Phagemid consist of
A. plasmid vector carrying λ phage's cos site
B. plasmid vector carrying λ attachment (λ att) site
C. plasmid vector carrying origin of replication of λ phage only
D. plasmid vector carrying origin of replication of plasmid only
Question 20
Selectable markers are the genes which code for resistance to _______
A. disease
B. phages
C. antibiotics
D. foreign entity
Question 21
Plasmid incompatibility is
A. inability of a plasmid to grow in the host
B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
C. both (a) and (b)
D. none of the above
Question 22
Phagemid vectors are
A. combination of plasmid and phage λ
B. combination of phages and cosmid
C. phages carrying properties of plasmids
D. All of the above
Question 23
Cos site of the cosmids
A. consists of 12 bases
B. helps whole genome in circularization and ligation
C. both (a) and (b)
D. contains cleavage site
Question 24
The process by which a foreign DNA is introduced into bacteria is called ______
A. amplification
B. transformation
C. infection
D. digestion
Question 25
λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
A. 6-7 kb
B. 1-2 kb
C. 40-44 kb
D. 20-23 kb
Question 26
Plasmids which are maintained as multiple copy number per cell are known as
A. stringent plasmids
B. relaxed plasmids
C. cryptic plasmids
D. none of these
Question 27
pBR 322 has/have which of the following selection marker(s)?
A. Ampr
B. Tetr
C. Both (a) and (b)
D. Kanr
Question 28
Which of the following is not true about phagemid?
A. Contain functional origin of replication of the plasmid and λ phage
B. May be propagated as a plasmid or as phage in appropriate strain
C. Contain λ att site
D. Can only be propagated as phage
Question 29
Which organism can transfer ‘T-DNA’ within plants?
A. Agrobacterium tumifaciens
B. E.coli
C. Aspergillus niger
D. S. typhi
Question 30
Which plasmid of Agrobacterium tumifaciens leads to tumor formation in dicots?
A. F plasmid
B. Ti
C. pUC
D. pBR
Question 31
M 13 is an example of
A. filamentous phage
B. single stranded DNA vector
C. Both A and B
D. plasmid
Question 32
P1 cloning vector is the example of
A. plasmid
B. cosmid
C. bacteriophage
D. phagemid
Question 33
Inserted DNA in λ gt 11 can be expressed as
A. β-galactosidase fused protein
B. free protein in the cytoplasm
C. free protein that is secreted out
D. All of the above
Question 34
Cosmid vectors are
A. plasmids that contain fragment of λ DNA including the cos site
B. phages that lack cos site
C. plasmids that have no selection marker
D. cryptic plasmids
Question 35
Conjugative plasmids
A. exhibit antibiotic resistance
B. do not exhibit antibiotic resistance
C. carry transfer genes called the tra genes
D. do not carry transfer genes
Question 36
pBR 322 has/have which of the following selection marker(s)?
A. Amp^r
B. Tet^r
C. Both A and B
D. Kan^r
Question 37
Plasmid incompatibility is
A. inability of a plasmid to grow in the host
B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
C. Both A and B
D. None of these
Question 38
Stuffer is
A. the right arm of the vector DNA
B. the left arm of the vector DNA
C. central fragment of the vector DNA
D. none of the above
Question 39
Maximum size of foreign DNA that can be inserted into an insertion vector is
A. 35 kb
B. 18 kb
C. 50 kb
D. 27 kb
Question 40
P1 cloning vector allow cloning of DNA of the length of
A. 100 kbp
B. 50 kbp
C. 20 kbp
D. 10 kbp
Question 41
What may complicate the process of gene cloning within the cell?
A. One recognition site
B. Foreign DNA
C. More than one recognition site
D. Antibody
Question 42
λ ZAP vector is an example of
A. phage
B. phagemid
C. cosmid
D. plasmid
Question 43
M 13 is an example of
A. filamentous phage
B. single stranded DNA vector
C. both (a) and (b)
D. plasmid
Question 44
EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
A. 6-7 kb
B. 15-25 kb
C. 40-44 kb
D. 1-2 kb
Question 45
Charon 34 and Charon 35 can clone DNA upto
A. 1-2 kb
B. 6-7 kb
C. 9-20 kb
D. 30-35 kb
Question 46
Charon vectors are different from EMBL vectors because
A. they have more extensive range of restriction targets with in their polylinkers.
B. physical separation of lambda arm from central fragment is required
C. both (a) and (b)
D. physical separation of lambda arm from central fragment is not required
Question 47
Viruses which infect bacteria are called ______
A. bacteria
B. archaea
C. pUC
D. bacteriophages