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Cloning Vectors MCQ Questions & Answers

Cloning Vectors MCQs : This section focuses on the "Cloning Vectors". These Multiple Choice Questions (MCQs) should be practiced to improve the Cloning Vectors skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

Plasmids and ________ have the ability to replicate within bacterial cells independent of the control of chromosomal DNA.

A. bacteriophages
B. fragments
C. bacteria
D. clones

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Question 2

EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to

A. 6-7 kb
B. 15-25 kb
C. 40-44 kb
D. 1-2 kb

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Question 3

Cosmid vectors are used for

A. cloning small fragments of DNA
B. cloning large fragments of DNA
C. cloning prokaryotic DNA only
D. cloning eukaryotic DNA only

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Question 4

Charon vectors are different from EMBL vectors because

A. they have more extensive range of restriction targets with in their polylinkers.
B. physical separation of lambda arm from central fragment is required
C. Both A and B
D. physical separation of lambda arm from central fragment is not required

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Question 5

Stuffer is

A. the right arm of the vector DNA
B. the left arm of the vector DNA
C. central fragment of the vector DNA
D. none of the above

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Question 6

M 13 is an example of

A. filamentous phage
B. single stranded DNA vector
C. Both A and B
D. plasmid

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Question 7

P1 cloning vector is the example of

A. plasmid
B. cosmid
C. bacteriophage
D. phagemid

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Question 8

Maximum size of foreign DNA that can be inserted into an insertion vector is

A. 35 kb
B. 18 kb
C. 50 kb
D. 27 kb

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Question 9

Phagemid vectors are

A. combination of plasmid and phage λ
B. combination of phages and cosmid
C. phages carrying properties of plasmids
D. All of the above

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Question 10

Selectable markers are the genes which code for resistance to _______

A. disease
B. phages
C. antibiotics
D. foreign entity

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Question 11

Charon 34 and Charon 35 are the examples of

A. plasmid vector
B. cosmid vector
C. phage vector
D. phagemid vector

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Question 12

Difference between λ gt 10 and λ gt 11 vectors is that

A. λ gt 11 is an expression vector
B. λ gt 10 is an expression vector
C. λ gt 10 is a replacement vector
D. λ gt 11 is a replacement vector

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Question 13

Conjugative plasmids

A. exhibit antibiotic resistance
B. do not exhibit antibiotic resistance
C. carry transfer genes called the tra genes
D. do not carry transfer genes

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Question 14

Plasmids which are maintained as limited number of copies per cell are known as

A. stringent plasmids
B. relaxed plasmids
C. cryptic plasmids
D. all of these

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Question 15

Plasmids which are maintained as multiple copy number per cell are known as

A. stringent plasmids
B. relaxed plasmids
C. cryptic plasmids
D. none of these

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Question 16

Insertion of recombinant DNA within the gene encoding for β–galactosidase leads to ________

A. amplification
B. transformation
C. insertional inactivation
D. cloning

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Question 17

Which organism can transfer ‘T-DNA’ within plants?

A. Agrobacterium tumifaciens
B. E.coli
C. Aspergillus niger
D. S. typhi

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Question 18

The sequence of DNA from where replication starts is called _______

A. selectable marker
B. origin of replication
C. ter sequence
D. genetic sequence

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Question 19

λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to

A. 6-7 kb
B. 1-2 kb
C. 40-44 kb
D. 20-23 kb

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Question 20

Plasmid incompatibility is

A. inability of a plasmid to grow in the host
B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
C. both (a) and (b)
D. none of the above

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Question 21

λ ZAP vector is an example of

A. phage
B. phagemid
C. cosmid
D. plasmid

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Question 22

pBR 322 has/have which of the following selection marker(s)?

A. Ampr
B. Tetr
C. Both (a) and (b)
D. Kanr

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Question 23

Cryptic plasmids

A. do not exhibit any phenotypic trait
B. exhibit many phenotypic traits
C. exhibit one phenotypic traits
D. exhibit antibiotic resistance

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Question 24

P1 cloning vector allow cloning of DNA of the length of

A. 100 kbp
B. 50 kbp
C. 20 kbp
D. 10 kbp

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Question 25

Cosmid vectors are

A. plasmids that contain fragment of λ DNA including the cos site
B. phages that lack cos site
C. plasmids that have no selection marker
D. cryptic plasmids

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Question 26

________ is an example of antibiotic.

A. Virus
B. Restriction endonuclease
C. RNA
D. Kanamycin

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Question 27

Charon vectors are different from EMBL vectors because

A. they have more extensive range of restriction targets with in their polylinkers.
B. physical separation of lambda arm from central fragment is required
C. both (a) and (b)
D. physical separation of lambda arm from central fragment is not required

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Question 28

Phagemid consist of

A. plasmid vector carrying λ phage's cos site
B. plasmid vector carrying λ attachment (λ att) site
C. plasmid vector carrying origin of replication of λ phage only
D. plasmid vector carrying origin of replication of plasmid only

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Question 29

Inserted DNA in λ gt 11 can be expressed as

A. β-galactosidase fused protein
B. free protein in the cytoplasm
C. free protein that is secreted out
D. All of the above

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Question 30

Select the wrong statement about plasmids?

A. It is extrachromosomal
B. It is double stranded
C. Its replication depends upon host cell
D. It is closed and circular DNA

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Question 31

Size of the DNA that can be packaged into a λ phage is

A. 50 kb
B. 35-53 kb
C. 40-50 kb
D. any size

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Question 32

A plasmid can be considered as a suitable cloning vector if

A. it can be readily isolated from the cells
B. it possesses a single restriction site for one or more restriction enzymes
C. insertion of foreign DNA does not alter its replication properties
D. All of the above

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Question 33

Charon 34 and Charon 35 can clone DNA upto

A. 1-2 kb
B. 6-7 kb
C. 9-20 kb
D. 30-35 kb

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Question 34

Maximum size of foreign DNA that can be inserted into a replacement vector is

A. 25-30 kb
B. 18-20 kb
C. 20-25 kb
D. 40-50 kb

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Question 35

Cosmids lack

A. genes coding for viral proteins
B. origin of replication
C. marker genes coding for replication
D. cleavage site for the insertion of foreign DNA

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Question 36

Cos site of the cosmids

A. consists of 12 bases
B. helps whole genome in circularization and ligation
C. Both A and B
D. contains cleavage site

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Question 37

Cos site of the cosmids

A. consists of 12 bases
B. helps whole genome in circularization and ligation
C. both (a) and (b)
D. contains cleavage site

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Question 38

Stuffer is

A. the right arm of the vector DNA
B. the left arm of the vector DNA
C. central fragment of the vector DNA
D. None of these

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Question 39

Viruses which infect bacteria are called ______

A. bacteria
B. archaea
C. pUC
D. bacteriophages

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Question 40

Single stranded vectors are useful

A. for sequencing of cloned DNA
B. for oligonucleotide directed mutagenesis
C. for probe preparation
D. All of the above

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Question 41

Plasmid incompatibility is

A. inability of a plasmid to grow in the host
B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
C. Both A and B
D. None of these

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Question 42

Which plasmid of Agrobacterium tumifaciens leads to tumor formation in dicots?

A. F plasmid
B. Ti
C. pUC
D. pBR

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Question 43

M 13 is an example of

A. filamentous phage
B. single stranded DNA vector
C. both (a) and (b)
D. plasmid

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Question 44

pBR 322 has/have which of the following selection marker(s)?

A. Amp^r
B. Tet^r
C. Both A and B
D. Kan^r

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Question 45

What may complicate the process of gene cloning within the cell?

A. One recognition site
B. Foreign DNA
C. More than one recognition site
D. Antibody

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Question 46

Which of the following is not true about phagemid?

A. Contain functional origin of replication of the plasmid and λ phage
B. May be propagated as a plasmid or as phage in appropriate strain
C. Contain λ att site
D. Can only be propagated as phage

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Question 47

The process by which a foreign DNA is introduced into bacteria is called ______

A. amplification
B. transformation
C. infection
D. digestion

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