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Cre Lox Excision MCQ Questions & Answers

Cre Lox Excision MCQs : This section focuses on the "Cre Lox Excision". These Multiple Choice Questions (MCQs) should be practiced to improve the Cre Lox Excision skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

Choose the correct statement for self cleavage reaction.

A. It is nucleophilic attack reaction by 2’ hydroxyl of RNA
B. It is nucleophilic attack reaction by 3’ hydroxyl of RNA
C. It is nucleophilic attack reaction by 2’ hydroxyl of DNA
D. It is nucleophilic attack reaction by 3’ hydroxyl of DNA

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Question 2

Choose the incorrect statement for cre-lox excision.

A. The chromosomal copy of the target gene replaces the target gene flanked by loxP sites
B. The second step is supply of Cre recombinase
C. Integration of cre takes place under a controllable promoter followed by induction of the promoter
D. Induction results in expression of cre, recombination along loxP sites and excision of the sequence between

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Question 3

Cre can be introduced by crossing it with a strain containing the gene or by infection with virus containing it.

A. True
B. False

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Question 4

Excision of DNA flanked by loxP sequences is also known as _________

A. subtle excision
B. croxing
C. floxing
D. sequence specific excision

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Question 5

Hepatitis delta virus capable of self cleavage.

A. True
B. False

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Question 6

How much effect is there on the surrounding genes by the loxP sequence which is left after recombination has taken place?

A. Little effect
B. No effect
C. Huge effect
D. It depends on the nature of the surrounding gene

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Question 7

RNA molecule with catalytic activity is termed as________

A. ribosomes
B. catalytic RNA
C. reactive RNA
D. ribozyme

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Question 8

Self cleavage reaction can take place in?

A. DNA
B. RNA
C. Both DNA and RNA
D. Can take place in both but is preferred in DNA

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Question 9

The ability to control the expression of cre allows controlling what?

A. recombination
B. replication
C. excision
D. packaging

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Question 10

The ________ protein of bacteriophage P1 mediates site-specific recombination at a 34 bp sequence, loxP.

A. cre recombinase
B. gene II
C. gene IV
D. gene VIII

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