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Downstream Processing MCQ Questions & Answers

Downstream Processing MCQs : This section focuses on the "Downstream Processing". These Multiple Choice Questions (MCQs) should be practiced to improve the Downstream Processing skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

Which of the operation does not come under upstream processing?

A. Media preparation
B. Inoculum development
C. Effluent treatment
D. Storage of raw material

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Question 2

Selectivity can be expressed as

A. ratio of the distribution coefficients in different solute
B. mass fraction of the solute in the extract phase at equilibrium
C. mass fraction of the solute in the raffinate phase
D. ratio of the mass fraction of the solute in the extract phase to the raffinate phase

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Question 3

Maintenance cost varies from

A. 0-4% of the capital cost
B. 12-16% of the capital cost
C. 4-8% of the capital cost
D. 8-12% of the capital cost

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Question 4

Chromatography is based on the

A. different rate of movement of the solute in the column
B. separation of one solute from other constituents by being captured on the adsorbent
C. different rate of movement of the solvent in the column
D. any of the above

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Question 5

If the solute passes through membrane freely, the the rejection coefficient (s) is

A. 1
B. 0
C. >1
D. <1

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Question 6

The efficiency of cell disruption in a bead mill depends on the

A. concentration of the cells
B. amount and size of beads
C. type and rotational speed of agitation
D. All of the above

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Question 7

A common filter medium is the cloth filter generally made of

A. canvas
B. synthetic fabrics
C. metal or glass fiber
D. All of the above

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Question 8

For cost analysis in a fermentor system, the depreciation cost varies from

A. 5-6% of the capital cost
B. 6-7% of the capital cost
C. 6-10% of the capital cost
D. 8-10% of the capital cost

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Question 9

The conventional filtration involves the separation of large particles generally

A. dp>5μm
B. dp>10μm
C. dp>15μm
D. dp>20μm

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Question 10

The correlation often employed to correlate adsorption data for protein is (where Ymax is the maximum amount of solute adsorbed per mass of adsorbent, X is the mass fraction of solute in the diluent phase in solute-free basis, KL is a constant and Y is the equilibrium value of the mass of solute adsorbed per mass of adsorbent)

A. Y = YmaxX/(KL+X)
B. Y = Ymax/(KL+X)
C. Y = YmaxX/KL
D. Y = Ymax- X/ (KL+X)

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Question 11

The effectiveness of a solvent can be measured by the

A. distribution coefficients
B. selectivity
C. both (a) and (b)
D. diffusivity

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Question 12

Micro filtration refers to the separation of suspended material such as bacteria by using a membrane with spore sizes of

A. 0.02 to 10μm
B. 1-10A°
C. 20-30μm
D. 10-200A°

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Question 13

The disk centrifuge is the type of centrifuge used most often for bio separations due to its

A. continuous operation
B. lesser cost
C. higher speed
D. ease in operation

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Question 14

The purity of a solute collected between two times t1 and t2 during chromatographic separation can be calculated as

A. amount of solute eluted - amount of impurity eluted
B. amount of solute eluted / amount of impurity eluted
C. amount of solvent eluted + amount of impurity eluted
D. amount of solvent eluted / amount of impurity eluted

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Question 15

For the extractions operation, the selectivity should be

A. > 1
B. < 1
C. 1
D. zero

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Question 16

In antibiotic manufacturing processes, the fermentation time ranges from

A. 2-3 weeks
B. 1-2 weeks
C. 4-5 weeks
D. 2-4 weeks

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Question 17

Concentration polarization can be reduced further by

A. pre filtering the solution
B. reducing the flow rate per unit membrane surface area
C. back washing periodically
D. All of the above

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Question 18

The stage wise operation of adsorption is called

A. contact filtration
B. conventional adsorption
C. affinity adsorption
D. ion exchange

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Question 19

The effectiveness of a solvent can be measured by the

A. distribution coefficients
B. selectivity
C. Both A and B
D. diffusivity

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Question 20

Filtration leaf test results indicate that the filtration rate of a protein product is 100 dry lbs/(ft2 hr). What size production filter would be required to obtain 100 dry lbs of filter cake/hr?

A. 1.54 ft2
B. 3.08 ft2
C. 65 ft2
D. 65 lbs/ ft2.hr

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Question 21

An ion exchange resin is composed of

A. polymeric network
B. ionic functional groups
C. counter ions
D. All of the above

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Question 22

Which of the following is considered as a pretreatment to the biological feeds?

A. Heating to denature the proteins
B. Addition of filter aids to increase the porosity
C. Addition of electrolytes
D. All of the above

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Question 23

The optimum wet solid content for the cell suspension for a bead mill is typically somewhere between

A. 5-15% by volume
B. 15-30% by volume
C. 30-60% by volume
D. 60-90% by volume

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Question 24

The efficiency of cell disruption in a bead mill depends on the

A. concentration of the cells
B. amount and size of beads
C. type and rotational speed of agitation
D. all of these

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Question 25

Start up expenses in a fermentation industry varies from

A. 0-5% of the capital cost
B. 10-15% of the capital cost
C. 5-10% of the capital cost
D. 15-20% of the capital cost

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Question 26

A common filter medium is the cloth filter generally made of

A. canvas
B. synthetic fabrics
C. metal or glass fiber
D. all of these

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Question 27

Conventional adsorption is a

A. reversible process
B. irreversible process
C. either reversible or irreversible
D. none of these

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Question 28

An ion exchange resin is composed of

A. polymeric network
B. ionic functional groups
C. counter ions
D. all of these

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Question 29

The solvent flux across the membrane can be expressed as (where A is the area, Lp is the membrane permeability, σ is the reflection point, Δp is applied pressure and Δπ is the osmotic pressure)

A. J = Lp(Δp - σΔπ)
B. J/A = Lp(Δp - σΔπ)
C. J = Lp/σΔπ
D. J/A = Lp/Δp

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Question 30

Which of the following is not the physical method for the cells rupturing?

A. Milling
B. Homogenization
C. Ultrasonication
D. Enzymatic digestion

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Question 31

Assuming the laminar flow across the filter, the rate of filtration (dVf/dt) can be expressed as a function of pressure drop Dp by the modified D'Arcy's equation as (where A is the area of filtering surface, K is the D'Arcy's filter cake permeability and L is the thickness of the filter cake)

A. (1/A) dVf/dt = Δp/μ(L/K)
B. dVf/dt = (1/A)Δp/μ(L/K)
C. (1/A) dVf/dt = Δp/μ LK
D. dVf/dt = (1/A)Δp/μ LK

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Question 32

A system which require less solvent and produces a more concentrated extract phase, is desired with a

A. large distribution coefficients
B. small distribution coefficients
C. very small distribution coefficients
D. constant distribution coefficients

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Question 33

Conventional adsorption is a

A. reversible process
B. irreversible process
C. either reversible or irreversible
D. None of the above

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Question 34

If the membrane rejects all solute, the rejection coefficient (s) is

A. 1
B. 0
C. >1
D. <1

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Question 35

A typical tubular centrifuge has a bowl of 2 to 5 inch in diameter and 9 to 30 inch in height with a maximum rotating speed of

A. 15,000 to 50,000 rpm
B. 50,000 to 100,000 rpm
C. 100,000 to 150,000 rpm
D. 150,000 to 200,000 rpm

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