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Gel Electrophoresis MCQ Questions & Answers

Gel Electrophoresis MCQs : This section focuses on the "Gel Electrophoresis". These Multiple Choice Questions (MCQs) should be practiced to improve the Gel Electrophoresis skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

Gel electrophoresis separates nucleic acid molecules based on ____________

A. charge on molecules
B. size of the molecules
C. nature of the molecules i.e. whether DNA or RNA
D. chemical properties of the nucleic acids

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Question 2

In SDS-PAGE, the protein sample is first

A. treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
B. fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.
C. treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
D. none of the above

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Question 3

Proteins can be visualized directly in gels by

A. staining them with the dye
B. using electron microscope only
C. measuring their molecular weight
D. none of these

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Question 4

In isoelectric focusing, proteins are separated on the basis of their

A. relative content of positively charged residue only
B. relative content of negatively charged residue only
C. size
D. relative content of positively and negatively charged residue

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Question 5

The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by

A. SDS-PAGE electrophoresis
B. gel filtration chromatography
C. combining information from (a)and (b)
D. isoelectric focusing

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Question 6

In a native PAGE, proteins are separated on the basis of

A. net negative charge
B. net charge and size
C. net positive charges size
D. net positive charge

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Question 7

In an SDS-PAGE

A. proteins are denatured by the SDS
B. proteins have the same charge-to-mass ratio
C. smaller proteins migrate more rapidly through the gel
D. all of the above

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Question 8

Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size.

A. TRUE
B. FALSE

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Question 9

Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in

A. both proteins migrate to the anode
B. histones migrate to the anode and myoglobin migrates to the cathode
C. histones migrate to the cathode and myoglobin migrates to the anode
D. both proteins migrate to the cathode

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Question 10

Proteins are separated in an SDS-PAGE experiment on the basis of their

A. positively charged side chains
B. molecular weight
C. negatively charged side chains
D. different isoelectric points

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Question 11

Often the DNA or RNA needs to be removed from the gel to the solid support. The process can be termed as ____________

A. Blotting
B. Transfer
C. Chromatography
D. DNA purification

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Question 12

In a gel filtration column

A. smaller proteins enter the beads more readily
B. large proteins elute first
C. both (a) and (b)
D. large proteins enter the beads more readily

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Question 13

A piece of DNA that is to be separated is crushed and soaked into the buffer. The majority of DNA diffuses, how can it be separated?

A. Filtration and centrifugation
B. Filtration or centrifugation
C. Allowing to sediment
D. By passing through a silica column

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