Gene Transfer in Plants MCQs : This section focuses on the "Gene Transfer in Plants". These Multiple Choice Questions (MCQs) should be practiced to improve the Gene Transfer in Plants skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Question 1
Liposomes mediated gene transfer in plants involves
A. plasmid DNA enclosed in a lipid bag
B. fusion of liposomes with protoplast
C. use of polyethylene glycol (PEG)
D. All of the above
Question 2
The transfer of intermediate vectors into Agrobacterium are brought about by
A. transformation
B. biparental mating
C. triparental mating
D. transduction
Question 3
Vir genes required for the T-DNA transfer and processing are located
A. on the T-DNA
B. outside the T-DNA region
C. on the plant genome
D. none of these
Question 4
Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?
A. It is carried out with the help of micromanipulator
B. The recipient cells are immobilized on artificial support or artificially bound to substarate
C. It employs needle with diameter greater than cell diameter
D. all of the above
Question 5
Plant transformation vectors based on Agrobacterium can generally be divided into
A. two vectors
B. four vectors
C. six vectors
D. eight vectors
Question 6
Co-integrating transformation vectors must include a region of homology in
A. the vector plasmid
B. the Ti-plasmid
C. between vector plasmid and Ti-plasmid
D. none of these
Question 7
T-DNA transfer and processing into plant genome requires products of which of the following genes?
A. vir A,B
B. vir G,C
C. vir D,E
D. All of these
Question 8
Advantage of microprojectile method over microinjection method for gene transfer in plants include
A. intact cells are used
B. method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
C. gene can be transferred to many cells simultaneously
D. All of the above
Question 9
Which technique is used to introduce genes into dicots?
A. electroporation
B. particle acceleration
C. microinjection
D. Ti plasmid infection
Question 10
In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
A. vir A
B. vir B
C. vir C
D. vir D
Question 11
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain
A. one set of sequence necessary for gene transfer
B. two sets of sequence necessary for gene transfer
C. three sets of sequence necessary for gene transfer
D. four sets of sequence necessary for gene transfer
Question 12
Microprojectile method of gene transfer in plants involves delivery of DNA
A. with the help of micromanipulator
B. with the help of bolistics
C. with the help of needles
D. any of the above
Question 13
Which of the following is not true about the direct repeats flanking T-DNA?
A. They are conserved between nopaline and octopine Ti-plasmids
B. These repeats are transferred intact to the plant genome
C. These are important in integration mechanism
D. all of the above
Question 14
Opine synthesis is the property
A. conferred to plant cells when it transformed by Agrobacterium tumefaciens
B. determined by the bacteria Agrobacterium tumefaciens
C. both (a) and (b)
D. of normal plant cells
Question 15
Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?
A. vir A and vir G
B. vir C and vir D
C. vir B and vir E
D. vir A and vir B
Question 16
In a plant tumour cell
A. complete Ti plasmid is incorporated in plant nuclear DNA
B. different parts of the Ti-plasmid are incorporated
C. only a small specific segment of callus T DNA is incorporated
D. may vary from plant to plant
Question 17
Opines are
A. amino acid derivatives found in tumor tissues
B. amino acid derivatives found in normal tissues
C. amino acid derivatives found in both normal as well as tumor tissues
D. None of the above
Question 18
In the liposome mediated gene transfer in plants, nucleic acids are
A. protected from nuclease digestion
B. stable in liposomes
C. Both A and B
D. not stable in liposomes
Question 19
Direct DNA uptake by protoplasts can be stimulated by
A. polyethylene glycol (PEG)
B. decanal
C. luciferin
D. all of these
Question 20
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
A. pBR 322 based plasmid vector
B. pRK 2013
C. pRN 3
D. all of these
Question 21
The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as
A. cos site
B. flanking sequences
C. border sequences
D. transfer sequences
Question 22
Agrobacterium tumefaciens is a
A. gram (+) bacteria
B. gram (-) bacteria
C. a fungi
D. a yeast
Question 23
Crown gall tissue
A. can be cultivated in vitro in absence of bacteria
B. retains tumorous properties when cultivated
C. both (a) and (b)
D. shows tumorous properties only in presence of bacteria
Question 24
The size of the virulent plasmid of Agrobacterium tumefaciens is
A. 40-80 kb
B. 80-120 kb
C. 140-235 kb
D. >235 kb
Question 25
Direct DNA uptake by protoplasts can be stimulated by
A. polyethylene glycol (PEG)
B. decanal
C. luciferin
D. All of the above
Question 26
The left segment of octopine T-DNA (TL) is necessary for
A. enzymes for agropine biosynthesis
B. tumour formation
C. conjugative transfer
D. all of these
Question 27
Integrated nopaline T-DNA occurs as
A. single segment
B. two segments
C. three segments
D. four segments
Question 28
Microinjection involves
A. injection of large amount of DNA
B. injection of DNA into bigger cells
C. injection with needle having diameter greater than cell diameter
D. all of the above
Question 29
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
A. pRK 2013
B. pRN 3
C. either (a) or (b)
D. none of these
Question 30
Which of the following is true about T DNA?
A. Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
B. Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
C. Integration of T DNA occurs at two specific sites in the plant nuclear DNA
D. Integration of T DNA occurs at one site that may be random in the plant nuclear DNA
Question 31
Virulence trait of Agrobacterium tumefaciens is borne on
A. chromosomal DNA
B. tumour inducing plasmid DNA
C. both chromosomal and plasmid DNA
D. cryptic plasmid DNA
Question 32
Which of the following is not true about the helper plasmids?
A. These can replicate in Agrobacterium
B. These help in the mediating conjugation of intermediate vectors
C. These can't replicate in Agrobacterium
D. All of the above
Question 33
The right segment of octopine T-DNA (TR) is necessary for
A. enzymes for agropine biosynthesis
B. tumour formation
C. conjugative transfer
D. all of these
Question 34
Which of the following are used as selection marker for the cells transformed with Agrobacterium?
A. Neomycin phosphotransferase
B. Streptomycin phosphotransferase
C. Hygromycin phosphotransferase
D. any of the above
Question 35
On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of
A. 12 bp
B. 20 bp
C. 25 bp
D. 30 bp
Question 36
Opines that are present in crown gall tumour include
A. octopine
B. nopaline
C. agropine
D. all of these
Question 37
Integrated octopine T DNA occurs as
A. single segment
B. two segments
C. three segments
D. four segments
Question 38
In the liposome mediated gene transfer in plants, nucleic acids are
A. protected from nuclease digestion
B. stable in liposomes
C. both (a) and (b)
D. not stable in liposomes
Question 39
Which of the following is true about Agrobacterium tumefaciens?
A. It causes crown gall disease of plants
B. It infects gymosperms
C. It infects dicotyledonous angiosperms
D. All of the above
Question 40
Crown gall tissue
A. can be cultivated in vitro in absence of bacteria
B. retains tumorous properties when cultivated
C. Both A and B
D. shows tumorous properties only in presence of bacteria
Question 41
Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?
A. Acetosyringone
B. α-hydroxy syringone
C. Both A and B
D. None of the above
Question 42
Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?
A. Acetosyringone
B. α-hydroxy syringone
C. Both (a) and (b)
D. None of these
Question 43
Microprojectile method of gene transfer in plants involves delivery of DNA
A. with the help of micromanipulator
B. with the help of bolistics
C. with the help of needles
D. Any of these
Question 44
Co-integrating transformation vectors must include a region of homology in
A. the vector plasmid
B. the Ti-plasmid
C. between vector plasmid and Ti-plasmid
D. None of the above