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Glycolysis MCQ Questions & Answers

Glycolysis MCQs : This section focuses on the "Glycolysis". These Multiple Choice Questions (MCQs) should be practiced to improve the Glycolysis skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.

Question 1

Which of the following is not a mechanism for altering the flux of metabolites through the rate-determining step of a pathway?

A. Allosteric control of the enzyme activity
B. Diffusional coupling between adjacent active sites
C. Genetic control of the enzyme concentration
D. Covalent modification of the enzyme

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Question 2

The enzymes of glycolysis in a eukaryotic cell are located in the

A. intermembrane space
B. plasma membrane
C. cytosol
D. mitochondrial matrix

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Question 3

Whenever the cell’s ATP supply is depleted, which of the following enzyme’s activity is increased?

A. Hexokinase
B. Pyruvate kinase
C. Glucokinase
D. Phosphofructokinase-1

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Question 4

High concentration of glucose 6-phosphate is inhibitory to ___________

A. Hexokinase
B. Pyruvate kinase
C. Glucokinase
D. Phosphofructokinase-1

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Question 5

The net gain of ATP in glycolysis is _____ ATP

A. 16
B. 32
C. 4
D. 8

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Question 6

Phosphofructokinase, the major flux-controlling enzyme of glycolysis is allosterically inhibited and activated respectively by

A. ATP and PEP
B. AMP and Pi
C. ATP and ADP
D. Citrate and ATP

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Question 7

Glucose from the breakdown of glycogen is obtained in

A. the liver by phosphorolysis
B. the muscles by phosphorolysis
C. the muscles by hydrolysis
D. both (a) and (b)

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Question 8

Why does the glycolytic pathway continue in the direction of glucose catabolism?

A. There are essentially three irreversible reactions that act as the driving force for the pathway
B. High levels of ATP keep the pathway going in a forward direction
C. The enzymes of glycolysis only function in one direction
D. Glycolysis occurs in either direction

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Question 9

Which is considered as the universal pathway in a biological system?

A. Krebs cycle
B. Electron Transport System
C. Glycolysis
D. Photo Oxidation

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Question 10

For every one molecule of sugar glucose which is oxidized __________ molecule of pyruvic acid are produced.

A. 1
B. 2
C. 3
D. 4

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Question 11

The active form of glycogen phosphorylase is phosphorylated, while the dephosphorylation of which active form occurs?

A. Glycogen synthase
B. Glycogen semisynthase
C. Glycogen hydrolase
D. Glycogen dehydrogenase

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Question 12

When concentration of the reactants is higher than the equilibrium concentration then

A. the gibbs free energy will be positive
B. the gibbs free energy will be negative
C. more products will be formed
D. both (b) and (c)

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Question 13

The gateway reaction takes place under the enzyme pyruvate dehydrogenase.

A. True
B. False

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Question 14

Glycogen has

A. α-1,4 linkage
B. α-1,6 linkages
C. α-1,4 and α-1,6 linkages
D. α-1,4 and β-1,6 linkage

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Question 15

Which substrate is used in the last step of glycolysis?

A. Glyceraldehyde 3-phosphate
B. Pyruvate
C. Phosphoenolpyruvate
D. 1, 3-bisphosphoglycerate

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Question 16

Name the protein that helps pyruvate enter into the mitochondrial matrix.

A. Transport protein
B. Contractile protein
C. Adipose protein
D. Secretory protein

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Question 17

Which of the following is not true of glycolysis?

A. ADP is phosphorylated to ATP via substrate level phosphorylation
B. The pathway does not require oxygen
C. The pathway oxidizes two moles of NADH to NAD+ for each mole of glucose that enters
D. The pathway requires two moles of ATP to get started catabo-lizing each mole of glucose

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Question 18

Which of the following regulates glycolysis steps?

A. Phosphofructokinase
B. Hexose kinase
C. Pyruvate kinase
D. All of these

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Question 19

Cleavage of Fructose 1, 6-biophosphate yields ___________

A. Two aldoses
B. Two ketoses
C. An aldose and a ketose
D. Only a ketose

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Question 20

In glycolysis, one molecule of glucose is reduced to_______

A. fructose
B. pyruvate
C. phosphate
D. phosphoglycerate

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Question 21

Glycolysis converts ___________

A. Glucose into pyruvate
B. Glucose into phosphoenolpyruvate
C. Fructose into pyruvate
D. Fructose into phosphoenolpyruvate

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Question 22

The scientists that discovered glycolysis are ______

A. Embden, Meyerhof, and Parnas
B. Hans Kreb and John Dalton
C. Rudolf Virchow and Robert Brown
D. Robert Hooke only

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Question 23

Pyruvic acid and pyruvate are the same substance itself.

A. True
B. False

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Question 24

Which of the following enzyme catalyzes the first step of glycolysis?

A. Hexokinase
B. Pyruvate kinase
C. Glucokinase
D. Phosphofructokinase-1

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Question 25

What is the general term used for the anaerobic degradation of glucose to obtain energy?

A. Anabolism
B. Oxidation
C. Fermentation
D. Metabolism

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Question 26

During glycolysis, one NADH is equivalent to _______ number of ATP.

A. 4
B. 3
C. 2
D. 1

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Question 27

During glycolysis, the major energy generating step involves

A. pyruvate kinase
B. phosphoglycerate kinase
C. glyceraldehyde-3 -dehydrogenase
D. Phosphofructokinase

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Question 28

Glycolysis is also called ________

A. EMP pathway
B. FMR pathway
C. LMS pathway
D. OMS pathway

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Question 29

The amount of energy received from one ATP is

A. 76 kcal
B. 7.3 kcal
C. 760 kcal
D. 1000 kcal

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Question 30

During catabolism, only about 40% of the energy available from oxidizing glucose is used to synthesize ATP. Remaining 60%

A. is lost as heat
B. is used to reduce NADP
C. remains in the products of metabolism
D. is stored as fat.

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Question 31

In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

A. the techniques for isolating the phosphate donor are not refined enough
B. no such phosphate donor exists
C. the high-energy phosphate donor is very short-lived and difficult to isolate
D. None of the above

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Question 32

Dihydroxyacetone phosphate is rapidly and reversibly converted to ___________

A. Glyceraldehyde 3-phosphate
B. 1, 3-bis-phosphoglycerate
C. Fructose 1, 6-bisphosphate
D. Fructose 6-phosphate

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Question 33

A kinase is an enzyme that

A. removes phosphate groups of substrates
B. uses ATP to add a phosphate group to the substrate
C. uses NADH to change the oxidation state of the substrate
D. removes water from a double bond

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Question 34

Glycolytic pathway regulation involves

A. allosteric stimulation by ADP
B. allosteric inhibition by ATP
C. feedback, or product, inhibition by ATP
D. all of the above

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Question 35

In the glycogen synthase reaction, the precursor to glycogen is

A. glucose-6-P
B. UTP-glucose
C. UDP-glucose
D. glucose-1-P

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Question 36

The released energy obtained by oxidation of glucose is stored as

A. a concentration gradient across a membrane

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Question 37


A. activates fructose-1,6-bisphosphatase
B. activates phosphofructokinase
C. inhibits fructose-1,6-bisphosphatase
D. both (b) and (c)

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Question 38

The glycolytic pathway (glucose → 2 pyruvate) is found

A. in all living organisms
B. primarily in animals excluding particles
C. only in eukaryotes
D. only in yeast

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Question 39

ATP is from which general category of molecules?

A. Polysaccharides
B. Proteins
C. Nucleotides
D. Amino acids

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Question 40

The product formed in the first substrate level phosphorylation in glycolysis is ___________

A. Pyruvate
B. 3-phosphoglycerate
C. 1, 3-bisphosphoglycerate
D. 2-phosphoglycerate

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Question 41

Which of the following could act as an uncoupler of electron transport and ATP synthesis?

A. The Fo base-piece of ATP synthase (without the Fl subunit)
B. Dinitrophenol
C. neither (a) nor (b)
D. Both (a) and (b)

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