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Growth and Nutrition of Bacteria MCQ Questions & Answers

Growth and Nutrition of Bacteria MCQs : This section focuses on the "Growth and Nutrition of Bacteria". These Multiple Choice Questions (MCQs) should be practiced to improve the Growth and Nutrition of Bacteria skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

The period between inoculation of bacteria in a culture medium and beginning of multiplication is known as

A. stationary phase
B. log phase
C. lag phase
D. decline phase

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Question 2

Generation time is

A. time required for the population to double
B. time required for the initial adjustment
C. obtained by expression t/n, where t = time interval, n = number of generation
D. both (a) and (c)

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Question 3

Which of the following has more tolerance for acidic pH (lower pH)?

A. Yeast and moulds
B. Bacteria
C. E. coli
D. None of these

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Question 4

A microbe, which grows at temperatures above 95° C is most likely to be

A. an archaean
B. a fungus
C. a protozoan
D. None of these

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Question 5

Which of the following is not the characteristic of a growth curve?

A. Shows development of microbial population under relatively stable environmental conditions
B. Plotted with logarithmic numbers
C. Graphs numbers of microbes versus time
D. Each growth curve consists of four distinct phases

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Question 6

The total biomass of an organism will be determined by the nutrient present in the lowest concentration relative to the organism's requirements is a statement of

A. Liebig's law of the minimum
B. Shelford's law of tolerance
C. quorum sensing
D. Heisenberg's principle of uncertainty

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Question 7

Bacteria of genus Nitrosomonas use __________ as their electron source.

A. ammonia
B. H2S
C. succinate
D. light

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Question 8

Organisms such as lactobacilli that have elaborate requirements for specific nutrients i.e. vitamins and other growth promoting substances, are generally called as

A. fastidious hetrotrophs
B. chemo-lithotrophs
C. chemotrophs
D. photo-organotrophs

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Question 9

Nutrient content and biological structures are considered as

A. implicit factor for microbial growth
B. intrinsic factor for microbial growth
C. processing factor
D. None of the above

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Question 10

An organism has an optimal growth rate when the hydrogen ion concentration is very high. This organism is

A. osmotolerant
B. acidophile
C. neutrophile
D. aerotolerant anaerobe

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Question 11

The cell reproduction in bacteria may occur by

A. binary fission
B. budding
C. fragmentation
D. All of the above

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Question 12

All organisms require at least small amounts of carbondioxide, However, some can use CO2 as their sole source of carbon. Such organisms are termed as

A. autotrophs
B. phototrophs
C. chemotrophs
D. photo-organotrophs

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Question 13

An organism is completely dependent on atmospheric O2 for growth. This organism is

A. osmotolerant
B. acidophile
C. facultative anaerobe
D. obligate aerobe

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Question 14

A culture broth tube was very turbid at the bottom of the tube but clear at the top of the tube indicating that the

A. broth is sterile
B. organism can tolerate oxygen
C. organism cannot produce superoxide dismutase and/or catalase
D. organism should be grown in an anaerobic chamber

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Question 15

The average size of cells during the exponential phase of growth is

A. greater than the lag phase
B. lesser than the lag phase
C. equal to the lag phase
D. none of these

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Question 16

The reproduction by budding occur in

A. Rhodopseudomonas acidophila
B. Hyphomicrobium vulgare
C. Bacillus subtilis
D. Both (a) and (b)

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Question 17

The term obligate anaerobe refers to an organism that

A. doesn't use oxygen but tolerates it
B. is killed by oxygen
C. uses oxygen when present or grows without oxygen when oxygen is absent
D. prefers to grow without oxygen

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Question 18

Bacteria and fungi multiply best

A. below 16°C
B. between 16-38°C
C. above 38°C
D. None of these

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Question 19

The organism which obtain their energy from chemicals are designated as

A. prototrophs
B. chemotrophs
C. organotrophs
D. autotrophs

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Question 20

The cell reproduction in bacteria may occur by

A. binary fission
B. budding
C. fragmentation
D. all of these

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Question 21

The straightforward method of binary fission explains how bacteria

A. grow in nutrient agar
B. evolve
C. move
D. reproduce

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Question 22

Antimicrobial constituents for the microbial growth in foods are

A. intrinsic factor
B. extrinsic factor
C. both (a) and (b)
D. none of these

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Question 23

What is the primary source of food for marine life?

A. Phytoplankton
B. Zooplankton
C. Sea weed
D. Grass

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Question 24

Which of the following is the suitable temperature range for mesophiles?

A. 20-30°C
B. 25-40°C
C. >40°C
D. None of these

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Question 25

During exponential growth, the growth rate is

A. number of generations per unit time
B. reciprocal of generation time
C. both (a) and (b)
D. none of these

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Question 26

An organism that expends energy to grow in a habitat with a low water activity in order to maintain internal solute concentrations to retain water is

A. osmotolerant
B. acidophile
C. aerotolerant anaerobe
D. alkalophile

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Question 27

Quantitative measurement of bacterial growth can be carried out by measuring

A. cell count
B. cell mass
C. cell activity
D. all of these

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Question 28

The growth is normally expressed as __________ in turbidimetric measurement

A. cells per ml
B. cfu/ml
C. optical density
D. mg N2 /ml

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Question 29

Which of the following organisms typically get their carbon for biosynthesis from organic compounds?

A. Aerobic, glucose-respiring bacteria (aerobic respiration)
B. Ammonia-oxidizing bacteria (chemolithotrophic bacteria)
C. Photosynthetic cyanobacteria (phototrophic metabolism)
D. None of the above

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Question 30

The ability of Vibrio fischeri to produce bioluminescence chemicals only when a certain population density has been reached is an example of

A. Liebig's law of the minimum
B. Shelford's law of tolerance
C. quorum sensing
D. Heisenberg's principle of uncertainty

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Question 31

The fragmentation occurs in

A. Streptomyces species
B. Nocardia species
C. Bacillus subtilis
D. None of these

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Question 32

A synergistic effect is observed when

A. one organism removes an inhibitory substance for another
B. one organism produces a growth factor utilized by another
C. microbial alteration of pH, aw, Eh favours the growth of another
D. all of the above

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Question 33

Starvation proteins are produced by a culture during which of the following segments of the growth curve?

A. Lag phase
B. Exponential phase
C. Stationary phase
D. Death phase

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Question 34

Pseudomonas pseudoflava can grow as

A. chemo-lithotrophs
B. chemo-organotrophs
C. both (a) and (b)
D. none of these

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Question 35

Implicit factors

A. depend on developing microflora
B. can be synergistic
C. both (a) and (b)
D. none of the above

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Question 36

Implicit factors

A. depend on developing microflora
B. can be synergistic
C. Both A and B
D. None of the above

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Question 37

A spore differs from an actively replicating bacterium in that the spore

A. is produced during a process involving asymmetric division
B. is able to withstand more extreme conditions than the replicating cell
C. is metabolically inactive
D. All of the above

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Question 38

The organism which grows best above 45°C are called

A. psychrophilic
B. mesosphilic
C. thermophilic
D. any of these

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Question 39

The generation time of a culture that produces two generations per hour is

A. greater than that produces three generations per hour
B. lesser than that produces three generations per hour
C. equal to that produces three generations per hour
D. none of the above

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Question 40

Some organisms can use reduced inorganic compounds as electron donors and are termed as

A. lithotrophs
B. phototrophs
C. chemotrophs
D. photo-organotrophs

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Question 41

Generation time of Escherichia coli is

A. 20 minutes
B. 20 hours
C. 20 days
D. 200 hours

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Question 42

In the exponential phase, the cells and cell mass

A. first increases then decreases
B. decreases
C. are constant
D. double at a constant rate

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Question 43

The microorganisms that grow best in a low-oxygen environment is called a (n)

A. aerobe
B. anaerobe
C. facultative
D. microaerophile

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Question 44

The combination of low levels of NaCl, NaNO3 (sodium nitrate), and slightly acid pH can prevent multiplication and toxin formation of

A. Salmonella
B. S. aureus
C. C. botulinum
D. all of these

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Question 45

Lag phase is also known as

A. period of initial adjustment
B. transitional period
C. generation time
D. None of the above

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Question 46

The term facultative anaerobe refers to an organism that

A. doesn't use oxygen but tolerates it
B. is killed by oxygen
C. uses oxygen when present or grows without oxygen when absent
D. requires less oxygen than is present in air

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Question 47

Postgates assay technique is useful in determining if a cell is viable even though it is

A. incapable of cell division
B. too small to see
C. an obligate aerobe
D. a thermophile

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Question 48

A culture broth tube was very turbid at the surface but clear throughout the rest of the tube indicating that the

A. organism are aerobes
B. organism should be grown in an anaerobic chamber
C. organism cannot produce superoxide dismutase and/or catalase
D. organism cannot tolerate oxygen

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Question 49

The organism which grows best above 45°C are called

A. psychrophilic
B. mesosphilic
C. thermophilic
D. None of the above

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Question 50

What are the extrinsic factors for the microbial growth?

A. humidity
B. storage temperature
C. composition of gas phase
D. all of these

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Question 51

Lag phase is also known as

A. period of initial adjustment
B. transitional period
C. generation time
D. none of these

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Question 52

The reproduction by budding occur in

A. Rhodopseudomonas acidophila
B. Hyphomicrobium vulgare
C. Bacillus subtilis
D. Both A and B

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Question 53

Which of the following procedures uses a photocell to measure absorbance of a culture to regulate the flow of culture media?

A. Coulter Counter
B. Hemostat
C. Petroff-Hausser chamber
D. Trubidostat

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Question 54

Which of the following is used to grow bacterial cultures continuously?

A. Chemostat
B. Coulter Counter
C. Hemostat
D. Petroff-Hausser chamber

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Question 55

Exponential phase of growth curve of bacteria is of limited duration because of

A. rise in cell density
B. accumulation of toxic metabolites
C. exhaustion of nutrients
D. all of these

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Question 56

The term aerotolerant anaerobe refers to an organism that

A. doesn't use oxygen but can grow in the presence of oxygen
B. is killed by oxygen
C. requires less oxygen than is present in air
D. prefers to grow without oxygen

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Question 57

Organisms, using organic compounds as electron donors are called

A. lithotrophs
B. phototrophs
C. chemotrophs
D. organotrophs

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Question 58

Microaerophilic bacteria are those which require

A. 21 % oxygen for growth
B. low levels of oxygen for growth (lesser than O2 present in atmosphere)
C. oxygen for activation of enzymes
D. none of the above

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Question 59

The equation used to obtain bacterial population by binary fission is

A. N = No2n
B. log N/No = (0.3010) n
C. log(N/No) = nlog 2
D. All of these

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Question 60

Which of the following organisms typically get their carbon for biosynthesis from carbon dioxide?

A. Glucose-fermenting bacteria (fermentation)
B. Anaerobic, glucose-respiring bacteria (anaerobic respiration)
C. Aerobic, glucose-respiring bacteria (aerobic respiration)
D. Ammonia-oxidizing bacteria (chemolithotrophic bacteria)

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Question 61

Autotrophic bacteria are those which

A. make their own food
B. form a long chain glycocalyx
C. are highly susceptible to penicillin
D. produce a blue-green pigment

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Question 62

During exponential growth, the growth rate is

A. number of generations per unit time
B. reciprocal of generation time
C. Both A and B
D. None of these

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Question 63

The equation used to obtain bacterial population by binary fission is

A. N = No2n
B. log N/No = (0.3010) n
C. log(N/No) = nlog 2
D. All of the above

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