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Identification of Bacteria MCQ Questions & Answers

Identification of Bacteria MCQs : This section focuses on the "Identification of Bacteria". These Multiple Choice Questions (MCQs) should be practiced to improve the Identification of Bacteria skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

The gram-negative organisms is

A. Actinomyces
B. Bacillus
C. Clostridium
D. None of these

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Question 2

Catalase production is negative in which of the following?

A. Streptococcus
B. Salmonella
C. Proteus
D. Staphylococcus

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Question 3

Which of the following is a substitute for crystal violet used in gram-staining procedure?

A. Methylene blue
B. Bromocresol green
C. Safranin
D. Phenolpthalene

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Question 4

Which of the following inference(s) indicate(s) the ability of an organism to utilize citrate as a sole source of carbon in Simmon's citrate medium?

A. Blue colour
B. Appearance of growth
C. Both A and B
D. None of the above

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Question 5

The organisms that can be acid-fast stained is

A. Nocardia
B. Tubercle bacilli
C. Lepra bacilli
D. All of the above

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Question 6

The primary stain of Gram's method is

A. safranin
B. phenolpthalene
C. crystal violet
D. methyl red

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Question 7

At what concentration of sulphuric acid, Nocardia resists decolourization?

A. 0.5% sulphuric acid
B. 5% sulphuric acid
C. 10% sulphuric acid
D. 8% sulphuric acid

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Question 8

Which of the following is commonly used as Gram's decolouriser?

A. Ethyl alcohol
B. Methyl alcohol
C. Acetone
D. A mixture of ethyl alcohol and acetone

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Question 9

Production of acetoin can be detected by which of the following tests?

A. Citrate test
B. Voges-Proskauer test
C. Methyl red test
D. Indole test

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Question 10

The production of sufficient acid by fermentation of glucose leads to decrease in pH such that pH of the medium falls below 4.5. Which of the following test can detect it?

A. Indole test
B. Methyl red test
C. Citrate utilization test
D. Voges-Proskauer test

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Question 11

What is the colour of gram -postive bacteria on a gram staining?

A. Purple
B. Pink
C. Colourless
D. Green

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Question 12

What is the colour of gram-negative bacteria on a gram staining?

A. Purple
B. Pink
C. Green
D. Colourless

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Question 13

Which of the following is a primary stain for acid fast staining of mycobacteria?

A. Crystal violet
B. Carbol fuchsin
C. Geimsa
D. Methylene blue

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Question 14

The deinococci are gram-positive cocci and rods that are distinctive in their unusually great resistance to

A. radiation
B. desiccation
C. Both A and B
D. extreme temperatures

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Question 15

Which of the following shows a positive urease test?

A. Proteus
B. Klebsiella
C. Both A and B
D. None of these

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Question 16

Which of the following(s) is/ are oxidase-positive bacteria?

A. Vibrio
B. Neisseria
C. Pseudomonas
D. All of the above

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Question 17

What is the order of reagents used in the Gram stain?

A. Crystal violet, iodine, safranin, decolorizer
B. Crystal violet, iodine, decolorizer, safranin
C. Safranin, crystal violet, decolorizer, iodine
D. Crystal violet, safranin, iodine, decolorizer

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Question 18

Gram staining was developed accidentally in 1884 by

A. Alexander Fleming
B. Hans Christian Gram
C. Joseph Christian Gram
D. Robert Gram

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Question 19

Which of the following organisms can be demonstrated with silver impregnation method?

A. Vibrios
B. Salmonella
C. Spirochaetes
D. Shigella

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Question 20

Which of the following(s) show(s) positive results for phenylalanine deaminase test?

A. Providencia
B. Proteus
C. Morganella
D. All of these

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Question 21

The process of Gram staining is based on the ability of bacterial cell wall

A. to retain the safranin dye
B. to retain the crystal violet dye during solvent treatment
C. to retain part of both dyes
D. None of the above

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Question 22

The deinococci are gram-positive cocci and rods that are distinctive in their unusually great resistance to

A. radiation
B. desiccation
C. both (a) and (b)
D. extreme temperatures

View Answer

Question 23

The organisms that can be acid-fast stained is

A. Nocardia
B. Tubercle bacilli
C. Lepra bacilli
D. all of these

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Question 24

The process of Gram staining is based on the ability of bacterial cell wall

A. to retain the safranin dye
B. to retain the crystal violet dye during solvent treatment.
C. to retain part of both dyes
D. none of above

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Question 25

Which of the following inference(s) indicate(s) the ability of an organism to utilize citrate as a sole source of carbon in Simmon's citrate medium?

A. Blue colour
B. Appearance of growth
C. Both (a) and (b)
D. None of these

View Answer

Question 26

Which of the following shows a positive urease test?

A. Proteus
B. Klebsiella
C. Both (a) and (b)
D. None of these

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Question 27

Which of the following(s) is/ are oxidase-positive bacteria?

A. Vibrio
B. Neisseria
C. Pseudomonas
D. All of these

View Answer