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Media Required for Plant Cell Culture MCQ Questions & Answers

Media Required for Plant Cell Culture MCQs : This section focuses on the "Media Required for Plant Cell Culture". These Multiple Choice Questions (MCQs) should be practiced to improve the Media Required for Plant Cell Culture skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

In plant cell culture media, auxins and cytokinins are used in the range of

A. l-50μM
B. 50-100μM
C. 100-125μM
D. more than 125μM

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Question 2

Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

A. utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
C. utilization of both ammonium and nitrate result in rise in pH
D. utilization of both ammonium and nitrate result in drop in pH

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Question 3

Concentration of sucrose generally used in plant cell culture media is

A. 10-15 g/l
B. 20-30 g/l
C. 40-50 g/l
D. 60-70 g/l

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Question 4

Neutralized activated charcoal is occasionally added to young regenerating cultures to

A. remove toxic phenolics produced by the stressed plant cell
B. help to remove plants growth regulators introduced at an earlier stage
C. Both A and B
D. maintain the pH of the medium

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Question 5

Which of the following growth regulator cause plant cells to grow?

A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids

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Question 6

What is 'nurse' or conditioned medium?

A. It is the media full of growth factors used for the growth of cells
B. It is the medium added to nurse the callus culture
C. Both (a) and (b)
D. It is the liquid medium removed from the suspension of fast growing cells

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Question 7

What are the macronutrients used in plant cell culture medium?

A. N, P, K, S, Na
B. N, P, K, Ca, Cl
C. N, P, K, S, Ca
D. N, P, Ca, Na, Cl

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Question 8

Which of the following growth regulator is used to stimulate embryo or shoot development?

A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids

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Question 9

Which of the following growth regulator is added for short initiation during plant regeneration from callus?

A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids

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Question 10

Which of the following is not an auxin?

A. Indole acetic acid (IAA)
B. Naphthalenacetic acid (NAA)
C. zeatin
D. Indole butyric acid

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Question 11

What is 'nurse' or conditioned medium?

A. It is the media full of growth factors used for the growth of cells
B. It is the medium added to nurse the callus culture
C. Both A and B
D. It is the liquid medium removed from the suspension of fast growing cells

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Question 12

To maintain the pH of the culture

A. organic acid such as citric, fumaric, malic and succinic acid is used
B. synthetic buffers such as Tris, MES or HEPS are used
C. Both A and B
D. ammonium salts are used

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Question 13

Which of the following is an ethylene biosynthesis inhibitor?

A. Citric acid
B. Succinic acid
C. Activated charcoal
D. Silver thiosulphate

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Question 14

Very high sugar concentration (40-100 g/l) have been used

A. in specialized secondary metabolite production
B. to adjust the osmotic potential of the media in short term treatment for regeneration
C. Both (a) and (b)
D. none of these

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Question 15

Very high sugar concentration (40-100 g/l) have been used

A. in specialized secondary metabolite production
B. to adjust the osmotic potential of the media in short term treatment for regeneration
C. Both A and B
D. None of the above

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Question 16

Which is/are the disadvantage/(s) of using IAA in plant cell culture media?

A. It is unstable in solution
B. Gets easily oxidized
C. Conjugated to inactive form by plant cells
D. All of the above

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Question 17

Which is/are the naturally occurring plant auxins?

A. Indole acetic acid (IAA)
B. Naphthalenacetic acid (NAA)
C. 2,4-dichlorophenoxyacetic acid
D. All of the above

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Question 18

Which of the following is not true about nurse or conditioned medium?

A. It is liquid removed from the suspension of fast growing cells
B. It contains uncharacterized growth factor released by growing cells
C. It is used in the culture of regenerating protoplast
D. it is removed aseptically from the culture and is autoclaved before use

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Question 19

Which is the most common carbon source used in the plant cell culture media?

A. Sucrose
B. Glucose
C. Fructose
D. Maltose

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Question 20

To maintain the pH of the culture

A. organic acid such as citric, fumaric, malic and succinic acid is used
B. synthetic buffers such as Tris, MES or HEPS are used
C. both (a) and (b)
D. ammonium salts are used

View Answer

Question 21

Neutralized activated charcoal is occasionally added to young regenerating cultures to

A. remove toxic phenolics produced by the stressed plant cell
B. help to remove plants growth regulators introduced at an earlier stage
C. both (a) and (b)
D. maintain the pH of the medium

View Answer

Question 22

Which of the following is not a cytokinin?

A. 2,4-dichlorophenoxyacetic acid
B. 6 benzylaminopurine
C. Zeatin
D. Kinetin

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Question 23

Silver thiosulphate is added to culture medium as it helps to

A. maintain the pH
B. remove toxic phenolics from plant cells
C. prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.
D. All of the above

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Question 24

Which of the following growth regulator promote cell division?

A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids

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