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Microbial Metabolism MCQ Questions & Answers

Microbial Metabolism MCQs : This section focuses on the "Microbial Metabolism". These Multiple Choice Questions (MCQs) should be practiced to improve the Microbial Metabolism skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

In establishing proton gradient for chemiosmotic ATP generation by aerobic respiration the terminal electron acceptor is

A. nitrate
B. oxygen
C. sulfate
D. CO2

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Question 2

For each glucose molecule broken down, there are______________number of reduced coenzymes to be oxidized.

A. 12
B. 8
C. 6
D. 4

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Question 3

The acquisition energy by glucose fermentation requires

A. substrate-level phosphorylation
B. electron transport of electrons from NADH
C. long-chain fatty acid oxidation
D. the enzyme formic-hydrogen lyase

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Question 4

Standard free energy change (ΔG) can be expressed as

A. ΔG° = -RTlnkeq
B. ΔG° = RTlnkeq
C. ΔG° = R/Tlnkeq
D. ΔG° = -RT/lnkeq

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Question 5

Radioisotopes are frequently used in the study of cells. Assume a culture of E. coli is grown in a culture medium containing radioactive phosphorous. At the end of 48 hours, it is expected to find the radioactive label located in

A. enzymes
B. RNA
C. phospholipids
D. all of these

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Question 6

Free energy change (ΔG) of a reaction is referred as the amount of energy

A. liberated during reaction
B. taken up during reaction
C. liberated or taken up during reaction
D. None of these

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Question 7

TCA cycle functions in

A. catabolic reactions
B. anabolic reactions
C. amphibolic reactions
D. none of these

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Question 8

ATPase

A. synthesizes ATP, coupled to transfer of extracellular protons into the cell
B. extrudes protons from the cell coupled to the hydrolysis of ATP to ADP
C. is the enzyme that incorporates ATP into messenger RNA
D. carries out each of the reactions indicated in (a) and (b)

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Question 9

Entner - Doudoroff pathway is not found in

A. aerobic prokaryotes
B. anaerobic prokaryotes
C. Both (a) and (b)
D. eukaryotes

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Question 10

Digestive reactions where large molecules are broken down into smaller ones are referred as

A. anabolism
B. catabolism
C. metabolism
D. biosynthesis

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Question 11

The bacteriochlorophylls used by the anoxygenic bacteria have absorbance maxima located in the_____portion(s) of the spectrum.

A. green
B. blue
C. ultraviolet
D. infrared

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Question 12

Which of the following groups contain(s) many unique coenzymes, such as coenzyme M and coenzyme F420?

A. Sulfate-reducing bacteria
B. Methanotrophs (methane-oxidizing microbes)
C. Methanogens (methane-producing microbes)
D. Acetogens (acetigens; acetate-producing microbes)

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Question 13

Bacteriochlorophyll differs from chlorophyll in what way?

A. The chelated metal in bacteriochlorophyll is not Mg
B. There are chemical differences between the two chlorophyll in their side (R) groups
C. They have different absorption spectra
D. Both (b) and (c)

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Question 14

In an oxygenic photosynthesis, the green and the purple bacteria do not use which of the following one as an electron source?

A. H2O
B. H2
C. H2S
D. S (elemental sulphur)

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Question 15

In the passive diffusion, solute molecules cross the membrane as a result of

A. concentration difference
B. pressure difference
C. ionic difference
D. all of these

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Question 16

Assimilatory sulfate reduction involves the nucleotide __________ during the incorporation of H2S in the production of __________ .

A. ATP; methionine
B. ATP; cytosine
C. UTP; cytosine
D. GTP; cytosine

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Question 17

The chlorophyll molecules used by eucaryotes and cyanobacteria absorb radiant energy in the____________portion(s) of the visible spectrum.

A. red
B. green
C. red and blue
D. green and ultraviolet

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Question 18

Which of the following catalyze liberation of orthophosphate from organic P compounds and inorganic pyrophosphate ?

A. Alkaline phosphates
B. Oxidoreductase
C. Protease
D. Hydrogenase

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Question 19

Entner-Doudoroff pathway is found in

A. aerobic prokaryotes
B. anaerobic prokaryotes
C. both (a) and (b)
D. aerobic eukaryotes

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Question 20

The reaction, where small precursor molecules are assembled into larger organic molecules is referred as

A. anabolism
B. catabolism
C. metabolism
D. any of these

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Question 21

Aerobic catabolism of glucose yields how much energy (ATP synthesized) relative to glucose fermentation?

A. Slightly less
B. About the same
C. Twice as much
D. More than 10 times as much

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Question 22

In the passive diffusion, solute molecules cross the membrane as a result of

A. concentration difference
B. pressure difference
C. ionic difference
D. All of the above

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Question 23

Anoxygenic photosynthetic bacteria oxidize

A. water
B. oxgyen
C. sulfide
D. ammonia

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Question 24

High energy transfer compounds are capable of

A. accepting large amounts of free energy
B. transferring large amounts of free energy
C. measuring free energy
D. none of the above

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Question 25

If radioactive bicarbonate was supplied to bacterial cells, which were actively synthesizing fatty acids, it is expected to find the bulk of the radioactivity in

A. cellular bicarbonate
B. the fatty acids
C. the cytoplasmic membrane
D. nucleic acids

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Question 26

If ΔG of a chemical reaction is positive in value and keq is less than 1 then the chemical reaction will

A. proceed in reverse direction
B. proceeed in forward direction
C. not take place in any of the direction
D. none of these

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Question 27

Which of the following(s) is/are the products of the light reactions of photosynthesis?

A. ATP only
B. NADPH only
C. ATP and O2 only
D. ATP, NADPH, and O2

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Question 28

When acetate is the sole source of carbon for some microorganisms, the cycle which is used, is called

A. pentose phosphate pathway
B. glycolyic pathway
C. glyoxylate pathway
D. oxaloacetate pathway

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Question 29

The catabolic reaction, pentose-phosphate exists in

A. prokaryotic cells
B. eukaryotic cells
C. prokaryotic and eukaryotic cells both
D. none of these

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Question 30

Incorporation of atmospheric N2to NH4+ occurs via the process of

A. assimilatory nitrate reduction
B. transamination
C. deamination
D. nitrogen fixation

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Question 31

The role of bacteriophyll in an oxygenic photosynthesis is to

A. reduce ferridoxin directly
B. reduce NADP directly
C. use light energy to energize an electron
D. transfer electrons to an intermediate in the sulfide oxidation pathway

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Question 32

Radioisotopes are frequently used in the study of cells. Assume a culture of E. coli is grown in a culture medium containing radioactive sulphur. At the end of 48 hours, it is expected to find the radioactive label located in

A. DNA
B. enzymes
C. RNA
D. all of these

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Question 33

DAHP synthetase catalyzes the condensation of

A. erythrose-4-phosphate
B. phosphoenol pyruvate
C. Both A and B
D. phenylalanine

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Question 34

The mechanism of passive or facilitated diffusion require

A. metabolic energy
B. concentration of solute against an electrochemical gradient
C. accumulation of solute against an electrochemical gradient
D. accumulation or concentration of solute against an electrochemical gradient

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Question 35

In aerobic respiration, the terminal electron acceptor is

A. oxygen
B. nitrogen
C. hydrogen
D. nitrate

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Question 36

DAHP synthetase catalyzes the condensation of

A. erythrose-4-phosphate
B. phosphoenol pyruvate
C. both (a) and (b)
D. phenylalanine

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Question 37

The major route for incorporation of ammonia (NH4+) into organic compounds is via

A. reduction of pyruvate or alpha-ketoglutarate by enzymes
B. atmospheric nitrogen fixation
C. oxidation of pyruvate
D. all of these

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Question 38

High energy transfer compounds are capable of

A. accepting large amounts of free energy
B. transferring large amounts of free energy
C. measuring free energy
D. None of these

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Question 39

Which of the following is responsible for phosphate solubilization?

A. Streptococcus
B. Streptomyces
C. Bacillus
D. Clostridium

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Question 40

Most of the energy in aerobic respiration of glucose is captured by

A. substrate-level phosphorylation
B. electron transport of electrons from NADH
C. long-chain fatty acid oxidation
D. the enzyme formic-hydrogen lyase

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Question 41

Phosphate regulation has been observed in the production of

A. alkaloids
B. antibiotics
C. gibberelins
D. all of these

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Question 42

If ΔG of a chemical reaction has a negative value, the reaction

A. releases energy
B. requires energy
C. both (a) and (b)
D. none of these

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Question 43

Phosphate deregulated mutants are

A. less sensitive to phosphate regulation
B. moderately sensitive to phosphate regulation
C. highly sensitive to phosphate regulation
D. none of these

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Question 44

Which of the following does not produce oxygen as a product of photosynthesis?

A. Oak trees
B. Purple sulfur bacteria
C. Cyanobacteria
D. Phytoplankton

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Question 45

Phosphate is considered to restrict the induction of

A. primary metabolites
B. secondary metabolites
C. both (a) and (b)
D. none of these

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Question 46

The reactions of the cell that are carried out for capturing energy are called

A. catabolism
B. metabolism
C. anabolism
D. activation energy

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Question 47

The glyoxylate cycle is used by some microorganisms when___________ is the sole carbon source.

A. acetate
B. nitrate
C. carbon dioxide
D. all of these

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Question 48

The relationship between an oxidation-reduction potential difference and the standard free energy change is (where n is the number of moles of electron transferred, F= Faraday's constant and E°= standard oxidation-reduction potential difference)

A. ΔG° = -nFE°
B. ΔG° = nFE°
C. ΔG° = -nFlnE°
D. ΔG° = nFlnE°

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Question 49

As the electron flow through the chains, much of their free energy is conserved in the form of ATP. This process is called

A. oxidative phosphorylation
B. electromotive potential
C. dehydrogenations
D. none of these

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Question 50

The phosphate inhibition in the clavine formation with Claviceps SD58, can be counteracted by the addition of

A. alanine
B. methionine
C. tryptophan
D. lysine

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Question 51

During the carboxylation phase of the Calvin cycle, CO2 combines with

A. ribulose 1,5 - bisphosphate
B. phosphoglyceraldehyde
C. pyruvic acid
D. oxaloacetic acid

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Question 52

In order to get inorganic phosphorous into organic compounds, the phosphate ion is incorporated via

A. substrate level phosphorylation
B. oxidative phosphorylation
C. both (a) and (b)
D. DNA

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Question 53

If ΔG of a chemical reaction is positive in value and keq is less than 1 then the chemical reaction will

A. proceed in reverse direction
B. proceeed in forward direction
C. not take place in any of the direction
D. None of these

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Question 54

Which of the following nucleoside diphosphates is used most often in carbohydrate anabolism?

A. Uridine diphosphate
B. Adenosine diphosphate
C. Guanine diphosphate
D. Thymine diphosphate

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Question 55

Phosphate is considered to restrict the induction of

A. primary metabolites
B. secondary metabolites
C. Both A and B
D. None of these

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Question 56

Which of the following statement is correct?

A. Phosphate repression can not be eliminated by optimization of nutrient medium, deregulated medium must be used as production strains
B. Phosphate repression can be eliminated by optimization of nutrient medium, deregulated medium must be used as production strains
C. Phosphate repression can be eliminated by optimization of nutrient medium, regulated medium must be used as production strains
D. Phosphate repression can not be eliminated by optimization of nutrient medium, regulated medium must be used as production strains

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Question 57

Dolichol phosphate is

A. a complex lipid involved in docking vesicles with the plasma membrane
B. the anchor on which sugars assemble before transfer to proteins
C. a chaperone used in protein folding
D. a product of phospholipase C activation

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Question 58

Hexose monophosphate pathway is also known as

A. phosphogluconate pathway
B. oxaloacetate pathway
C. malate pathway
D. fumerate pathway

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Question 59

Nitrogen fixation is a process that requires

A. energy
B. an anaerobic environment
C. both (a) and (b)
D. an aerobic environment

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Question 60

The specific enzyme/(s) of the glyoxylate cycle is/are

A. isocitrate lyase
B. malate synthase
C. both (a) and (b)
D. anaplerotic

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Question 61

Radioisotopes are frequently used in the study of cells. Assume a culture of E. coli is grown in a culture medium containing radioactive phosphorous. At the end of 48 hours, it is expected to find the radioactive label located in

A. enzymes
B. RNA
C. phospholipids
D. All of the above

View Answer