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Microbial Recombination and Gene Transfer MCQ Questions & Answers

Microbial Recombination and Gene Transfer MCQs : This section focuses on the "Microbial Recombination and Gene Transfer". These Multiple Choice Questions (MCQs) should be practiced to improve the Microbial Recombination and Gene Transfer skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

The plasmid which makes the host more pathogenic is

A. F factors
B. Metabolic plasmid
C. Virulence plasmid
D. None of these

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Question 2

The term used for acquisition of naked DNA from its environment and its incorporation in their genome by a bacterium is

A. transformation
B. lysogenic conversion
C. conjugation
D. transduction

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Question 3

Which of the following is used by microbial genetisists as a tool?

A. Bacteriophage
B. Plasmids
C. Transposable elements
D. All of these

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Question 4

Which of the following statement(s) is/are true in regards to F+ x F- mating events?

A. DNA is transferred from F- to F+ cells
B. DNA is transferred from F+ to F- cells
C. No DNA is transferred because F- cells are unable to perform conjugation
D. No DNA is transferred because F+ cells are unable to perform conjugation

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Question 5

Which type of plasmid can exist with or without being integrated into the host's chromosome?

A. Medisome
B. Lisosome
C. Lysogen
D. Episome

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Question 6

The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

A. have Px but not the rest of the X coding region
B. have the promoter of lacZ or some other reporter gene
C. allow to monitor the expression of all genes with a promoter similar in sequence to Px
D. give the same information as from a microarray

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Question 7

R factors involved in plant-microbe interactions are

A. plant proteins
B. bacterial proteins
C. essential for transfer of DNA to plant cells
D. also called opines

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Question 8

F factor plasmids play a major role in

A. conjugation
B. replication
C. transduction
D. trasnscription

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Question 9

The plasmid-mediated properties is/are

A. fermentation of lactose
B. production of enterotoxin
C. resistance to antibiotics
D. All of the above

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Question 10

Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?

A. Mutagenic recombimation
B. Site-specific recombination
C. Replicative recombination
D. General recombination

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Question 11

What is the term used for a segment of DNA with one or more genes in the centre and the two ends carrying inverted repeat sequences of nucleotides?

A. Plasmid
B. Transposon
C. Insertion sequence
D. None of these

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Question 12

The term used for plasmids possessing both RTF and r determinants is

A. non self-transmissible plasmids
B. non conjugative plasmids
C. conjugative plasmids
D. none of the above

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Question 13

Who discovered transposons (jumping genes)?

A. Abelson
B. Harvey
C. McClintock
D. Griffith

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Question 14

What is term used for a bacterial cell that is able to take up naked DNA?

A. Complementary
B. Liable
C. Competent
D. Infected

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Question 15

The plasmids can be eliminated from a cell by the process known as

A. curing
B. breaking
C. fixing
D. expulsion

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Question 16

Which of the following type of recombination does not require homologous sequences and is utilized by mobile genetic elements that move about chromosomes?

A. Mutagenic recombimation
B. Site-specific recombination
C. Replicative recombination
D. General recombination

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Question 17

Which of the following plamids do not possess information for self transfer to another cell?

A. Cryptic plasmids
B. Conjugative plasmids
C. Non-conjugative plasmids
D. None of these

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Question 18

Which of the following genetic elements carry the genes required for integration into host chromosomes?

A. Replicon
B. Plasmids
C. Transposons
D. Tandons

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Question 19

Plasmid that carries genes encoding enzymes, which degrade substances such as aromatic compounds, pesticides or sugar are

A. F factors
B. metabolic plasmid
C. virulence plasmid
D. none of these

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Question 20

What is the term used for a segment of DNA with one or more genes in the centre and the two ends carrying inverted repeat sequences of nucleotides?

A. Plasmid
B. Transposon
C. Insertion sequence
D. none of the above

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Question 21

Penicillin resistance in staphylococci is acquired due to

A. conjugation
B. mutation
C. transformation
D. transduction

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Question 22

Which of the following term describes the relationship between a virus and host where no new viral particles are produced and the viral genome is replicated along with host chromosome?

A. Lysogeny
B. Lysis
C. Transformation
D. Conjugation

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Question 23

Which of the following statement can describe horizontal transfer?

A. The synthesis of protein in RNA
B. The transmission of genetic information from one independent, mature organism to another
C. The transmission of genetic information from parent to offspring
D. The synthesis of RNA from a DNA template

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Question 24

Recombination of virus genomes occurs

A. by transduction
B. by transription
C. simultaneous infection of a host cell by two viruses with homologous chromosomes
D. by transformation

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Question 25

Which of the following statement describes plasmids?

A. Another name for a protoplast
B. A complex membrane structure that covers the chromosome of bacteria
C. Small, circular DNA molecules that can exist independently of chromosomes commonly found in bacteria
D. None of these

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Question 26

When composite transposons are formed

A. a small deletion occurs in the transposase gene of an IS element
B. a small deletion occurs in the transposase gene of an IS element and plasmid is integrated
C. an IS element integrates with another IS element with the help of a plasmid
D. two IS elements integrate into a chromosome with only a small distance separating them

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Question 27

In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by

A. conjugal transfer by a self-transmissible plasmid
B. generalized phage transduction
C. natural transformation
D. none of the above

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Question 28

The correct term for the transfer of genetic material between bacteria in direct physical contact is

A. conjugation
B. transformation
C. replication
D. transduction

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Question 29

The chromosomal genes, possessing fertility factor is known as

A. R factor
B. F prime factor
C. HFr
D. F factor

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Question 30

The type of recombination that commonly occurs between a pair of homologous DNA sequences is,

A. mutagenic recombination
B. site-specific recombination
C. replicative recombination
D. general recombination

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Question 31

In lysogeny,

A. a bacteriophage transfers bacterial DNA
B. bacteria take up double stranded DNA from the environment
C. DNA-degrading enzymes in the extracellular medium would stop the process
D. a bacteriophage genome is integrated into the bacterial genome

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Question 32

The term used for plasmids possessing both RTF and r determinants is

A. non self-transmissible plasmids
B. non conjugative plasmids
C. conjugative plasmids
D. None of these

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Question 33

Inverted repeat sequences at each end and a gene encoding transposase is contained in which of the following transposable element?

A. Composite transposon
B. Insertion element
C. Virus
D. Plasmid

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Question 34

The transposase gene encodes an enzyme that facilitate

A. viral replication within a genome
B. general recombination
C. site-specific integration of transposable elements
D. none of the above

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Question 35

The transducing particles carry only specific portions of the bacterial genome in which of the following transduction?

A. Specialized transduction
B. General transduction
C. Abortive transduction
D. None of these

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Question 36

A microarray differs from a gene fusion in that, it

A. carries DNA segments from many different genes
B. is not constructed by cloning
C. gives direct measurement of mRNA level
D. All of the above

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Question 37

Diagnostic DNA probes have been developed for

A. Mycobacterium tuberculosis
B. Hepatitis B virus
C. Human immunodeficiency virus
D. All of the above

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Question 38

Which of the following is the cause for drug resistance in tuberculosis?

A. Mutation
B. Transduction
C. Transformation
D. Conjugation

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Question 39

The main difference between a self-transmissible and a mobilizable plasmid is that the self-transmissible plasmid

A. transfers both strands of the plasmid DNA
B. carries genes encoding the mating apparatus
C. transfers antibiotic resistance genes
D. usually has a transposon inserted into it

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Question 40

Which of the following transport bacterial DNA to other bacteria via bacteriophages?

A. Conjugation
B. Transduction
C. Transformation
D. Translation

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Question 41

In lysogeny

A. a bacteriophage transfers bacterial DNA
B. bacteria take up double stranded DNA from the environment
C. DNA-degrading enzymes in the extracellular medium would stop the process
D. a bacteriophage genome is integrated into the bacterial genome

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Question 42

The plasmid-mediated properties is/are

A. fermentation of lactose
B. production of enterotoxin
C. resistance to antibiotics
D. all of these

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Question 43

What information can be generated by interrupted mating experiments?

A. Levels of DNA homology
B. Bacterial genome maps
C. DNA nucleotide sequences
D. Proteomics of the bacteria

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Question 44

Which of the following statement describes plasmids?

A. Another name for a protoplast
B. A complex membrane structure that covers the chromosome of bacteria
C. Small, circular DNA molecules that can exist independently of chromosomes commonly found in bacteria
D. None of the above

View Answer