Monoclonal Antibodies MCQs : This section focuses on the "Monoclonal Antibodies". These Multiple Choice Questions (MCQs) should be practiced to improve the Monoclonal Antibodies skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Question 1
The approach (s), which is/are currently followed to produce human monoclonal antibodies, is/are known as
A. transformation of antigen specific B lymphocytes (EBV)
B. hybridization of 6-thioguanine-resistant human plasmacytoma with immune human lymphocytes
C. combination of EB Vand hybridoma techniques
D. All of the above
Question 2
Some cross reactions with monoclonal antibodies (MAbs) can occur. Unexpected cross reactions occur more frequently with
A. Ig MAbs
B. IgG
C. IgA
D. IgE
Question 3
Preliminary clinical results with a humanized antibody against the interleukin-2 receptor have suggested the
A. absence of human immune response against murine proteins (HAMA) response
B. presence of HAMA response
C. poor recognition of immunoglobulin, Ig constant regions
D. All of the above
Question 4
The cross linkage of antigens by antibodies is known as
A. agglutination
B. complement fixation
C. a cross reaction
D. All of the above
Question 5
In monoclonal antibody technology, tumor cells that can replicate endlessly are fused with mammalian cells that produce an antibody. The result of this cell fusion is a
A. hybridoma
B. myeloma
C. natural killer cell
D. lymphoblast
Question 6
In human B cells and T cells are matured in the
A. bone marrow and thymus respectively
B. lymph nodes and spleen respectively
C. bursa and thymus respectively
D. None of the above
Question 7
An example of mosaic antigen is
A. virus
B. bacteria
C. a hapten
D. protein
Question 8
T cells are the source of
A. interleukin
B. interferon
C. lymphotoxin
D. All of the above
Question 9
The primary B cell receptor is
A. IgD
B. IgG
C. IgA
D. IgE
Question 10
It is highly valued if the lymphocytes derived from the lymph node or tonsil tend to undergo fusion at
A. high frequencies
B. moderate frequencies
C. low frequencies
D. at no frequency
Question 11
Small simple molecules are
A. poor antigens
B. rich antigens
C. moderate antigens
D. heterophilic antigens
Question 12
The EBV-hybridoma technique
A. immortalizes the donor Bcells
B. facilitates the proliferation of antigen specific B cells
C. gives much higher hybridization frequencies
D. All of the above
Question 13
Helper T cells assist in the functions of
A. certain B cells B
B. certain T cells
C. certain B cells and other T cells
D. None of the above
Question 14
In immuno-inflammatory diseases such as hemolytic anaemia, eczema etc.,
A. T8 cells are greatly reduced
B. T8 cells are greatly increased
C. T4 cells are greatly reduced
D. T4 cells are greatly increased
Question 15
Which of the following is incorrect?
A. MAbs can be used to diagnose or treat diseases
B. MAbs can be used in tumor scanning
C. MAbs canbe used in cancer diagnosis
D. MAbs can not be used in such non-infectious diseases as those of endocrine system
Question 16
The antigen-specific lymphocytes can be immortalized by which of the following method?
A. Transfection with tumor derived DNA
B. Hybridization with a suitable lymphoid tumor cell
C. Transformation following infection by Epstein-Barr virus (EB V)
D. All of the above
Question 17
A cytokine that stimulates the activity of B and T cells is
A. lymphotoxin
B. interlukin-2
C. interlukin-1
D. All of the above
Question 18
Which type of cell actually secrets antibodies?
A. plasma cells
B. T cells
C. macrophages
D. dendritic cells
Question 19
Which of the following is correct?
A. rabbits do not make myelomas whereas mice are unable to synthesize antibodies
B. rabbits do not make myelomas whereas mice are able to synthesize antibodies
C. rabbits make myelomas whereas mice are unable to synthesize antibodies
D. rabbits make myelomas whereas mice are able to synthesize antibodies
Question 20
The Ig locus is about
A. 2/3rd of all hybridomas
B. l/3rd of all hybridomas
C. l/2nd of all hybridomas
D. 1/4th of all hybridomas
Question 21
A cytokine that stimulates the activity of B and T cells is
A. lymphotoxin
B. interlukin-2
C. interlukin-1
D. all of these
Question 22
Helper T cells assist in the functions of
A. certain B cells
B. certain T cells
C. certain B cells and other T cells
D. none of the above
Question 23
In human B cells and T cells are matured in the
A. bone marrow and thymus respectively
B. lymph nodes and spleen respectively
C. bursa and thymus respectively
D. none of these
Question 24
T cells are the source of
A. interleukin
B. interferon
C. lymphotoxin
D. all of these
Question 25
TC cells are important in controlling
A. virus infections
B. allergy
C. autoimmunity
D. all of these
Question 26
The approach (s), which is/are currently followed to produce human monoclonal antibodies, is/are known as
A. transformation of antigen specific B lymphocytes (EBV)
B. hybridization of 6-thioguanine-resistant human plasmacytoma with immune human lymphocytes
C. combination of EB Vand hybridoma techniques
D. all of these
Question 27
The cross linkage of antigens by antibodies is known as
A. agglutination
B. complement fixation
C. a cross reaction
D. all of these
Question 28
The hybrid cells can be propagated
A. in tissue culture
B. as ascites in peritoneal cavity of mice
C. both (a) and (b)
D. none of these
Question 29
Biosensors & Microarrays are diagnostic applications of monoclonal antibodies.
A. True
B. False
Question 30
Bispecific antibodies can bind with their Fab regions both to target antigen and to _____________
A. Other antibodies
B. An effector cell
C. Proteins around
D. Prostaglandins
Question 31
For how many weeks should you titer the Flow cytometry solution to get pure antigen?
A. 1 month
B. 3 weeks
C. 7 weeks
D. 2 weeks
Question 32
For which discovery did Georges Köhler and César Milstein share the noble prize in 1984?
A. Inventing genome sequencing
B. Discovery the structure of DNA
C. Discovery of B-cell cancer myeloma
D. Discovery of the process of producing monoclonal antibodies
Question 33
Fight against Bioterrorism is one of the clinical applications of monoclonal antibodies.
A. True
B. False
Question 34
MAbs act directly when binding to cancer-specific antigens and induce an immunological response to cancer cells.
A. True
B. False
Question 35
MAbs was modified for delivery of a toxin, radioisotope and __________
A. Enzymes
B. Hormones
C. Drugs
D. Cytokine
Question 36
MCA are antibodies that are non-identical.
A. True
B. False
Question 37
The cell fusion happens between spleen cells and _____________
A. Red blood cells
B. Cancer cell
C. Myeloma cells
D. Leukocytes
Question 38
What do we get when we fuse Spleen cells and Myeloma cells?
A. Hybridoma cells
B. Red blood cells
C. Killer cells
D. Cancer myeloma B-cell
Question 39
What do you add to Myeloma cells to receive HGPRT- myeloma cells?
A. 8 – Azaguanine
B. Azaguanine
C. Nitrogen flush
D. Carbon dioxide flush
Question 40
What had helped the study of the structure of antibodies in 1970?
A. APC
B. Red blood cells
C. Killer cells
D. Cancer myeloma B-cell
Question 41
What has become an important tool in biochemistry, molecular biology, and medicine?
A. DNA structure
B. Genome sequencing
C. MCA
D. PCR
Question 42
What is the clinical application of monoclonal antibodies?
A. Biosensors
B. Transplant rejection
C. Infectious disease
D. Purification of drugs
Question 43
What is the method to harvest monoclonal antibodies from the positive clones?
A. Subculture in the new medium
B. Bioreactors
C. One a single media plate
D. Tissue culture method
Question 44
Who invented the process of producing monoclonal antibodies in 1975?
A. Albert Einstein
B. Watson and Creek
C. Georges Köhler and César Milstein
D. Robert Hook