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Physical and Chemical Agents MCQ Questions & Answers

Physical and Chemical Agents MCQs : This section focuses on the "Physical and Chemical Agents". These Multiple Choice Questions (MCQs) should be practiced to improve the Physical and Chemical Agents skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

The lowest temperature that kills all microorganisms in a liquid suspension in 10 minutes is known as the

A. decimal reduction time
B. thermal death point
C. thermal death temperature
D. thermal death time

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Question 2

If a canning procedure is not properly followed, which type of microbe is most likely to grow in the canned food?

A. Obligate Aerobe
B. Acidophile
C. Mesophile
D. Obligate Anaerobe

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Question 3

Preservation of foods by using salts and sugars works by

A. raising pH
B. lowering osmotic pressure
C. creating a hypertonic environment
D. creating a hypotonic environment

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Question 4

The time in minutes at a specific temperature needed to kill a population of cells is the

A. decimal reduction time
B. thermal death point
C. thermal death temperature
D. F value

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Question 5

Which of the following methods will not sterilize?

A. Aqueous glutaraldehyde for 10 hours
B. Dry heat at 171°C for 1 to 2 hours
C. 121°C at 15 lb/in pressure for 15 to 20 minutes
D. 100°C boiling water for 30 minutes

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Question 6

Which of the following is most effective for sterilizing mattresses and plastic petri plates?

A. Chlorine
B. Ethylene oxide
C. Glutaraldehyde
D. Ultraviolet radiation

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Question 7

Which of the following is best to sterilize heat labile solutions?

A. Dry heat
B. Autoclave
C. Membrane filtration
D. Pasteurization

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Question 8

Which of the following is a limitation of the autoclave?

A. It takes too long to sterilize
B. It lacks the ability to inactivate viruses
C. It lacks the ability to kill endospores
D. It will destroy heat labile materials

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Question 9

Which of the following substances can sterilize?

A. Alcohol
B. Cetylpyridinium chloride
C. Ethylene oxide
D. Chlorine

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Question 10

Which of the following material is sterilized by heating at 160°C in a hot air oven for one hour?

A. Swab sticks
B. All-glass syringes
C. Oils and jellies
D. All of these

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Question 11

Which of the following articles can be sterilized in an autoclave?

A. Gloves
B. Culture media
C. Dressing material
D. All of these

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Question 12

Microbes can be removed from a liquid solution by the process of

A. filtration
B. freeze-drying
C. osmosis
D. desiccation

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Question 13

Which of the following does not kill endospores?

A. Autoclave
B. Incineration
C. Hot air sterilization
D. Pasteurization

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Question 14

Sweet and salty foods frequently don't require refrigeration to prevent spoilage because they have

A. insufficient nutrients
B. low pH
C. high concentration of solutes
D. toxic alkaline chemicals

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Question 15

The process of making an object free from living organisms including bacterial and fungal spores and viruses is known as

A. pasteurization
B. antisepsis
C. disinfection
D. sterilization

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Question 16

Ethylene oxide is used to destroy or kill which of the following microbes?

A. Bacteria
B. Spores
C. Fungi
D. All of these

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Question 17

Complete destruction of the microorganisms is commonly known as

A. disinfection
B. sterilization
C. antisepsis
D. none of these

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Question 18

Media containing spores and thermolabile constituents are sterilized by

A. pasteurization
B. UV radiation
C. dry heat
D. tyndalization

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Question 19

Milk is pasteurized in batch method by keeping it at

A. 63°C for 30 minutes
B. 72°C for 60 seconds
C. 73°C for 30 minutes
D. 72°C for 6 minutes

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Question 20

One drawback to the use of ultraviolet light as a sterilizing agent is its

A. failure to kill bacteria
B. failure to bacterial spores
C. failure to kill microbes in a closed environment
D. failure to kill microbes located in the center of an object

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Question 21

Which of the following disinfectants act by disrupting microbial membranes?

A. Cationic detergents
B. Halogens
C. Heavy metals
D. Aldehydes

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Question 22

Which of the following is/are vapour-phase disinfectant/s?

A. Formaldehyde
B. Ethylene oxide
C. Both (a) and (b)
D. None of these

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Question 23

Steam exposure of a material at 100°C for 20 minutes for three consecutive days is known as

A. inspissation
B. tyndallization
C. autoclaving
D. none of these

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Question 24

Which of the following process can be efficiently carried out by incineration?

A. Sterilization of scalpel blades and needles
B. Sterilization of all glass syringes
C. Sterilization of points of forceps
D. Destruction of contaminated materials

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Question 25

The organisms retained in the fluids filtered by Seitz filter is

A. Proteus
B. Staphylococcus
C. Clostridium
D. None of these

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Question 26

Which of the following items could be sterilized by dry heat sterilization?

A. Intravenous (IV) solution
B. Plastic IV bags
C. Glass pipettes
D. rubber gloves

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Question 27

Which of the following articles can be sterilized in an autoclave?

A. Gloves
B. Culture media
C. Dressing material
D. All of the above

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Question 28

For sterilization of which material gamma rays can be used?

A. Catheters
B. Plastic syringes
C. Canulas
D. None of these

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Question 29

Which disinfectant was the most effective against Salmonella"?

A. Phenol
B. Cetylpyridinium chloride
C. Hexachlorophene
D. Merthiolate

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Question 30

Iodophores are mixture of

A. iodine and aldehydes
B. iodine and surface active agents
C. iodine and alcohols
D. iodine and phenols

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Question 31

Which of the following tests is designed to simulate the natural conditions under which the disinfectants are used in hospitals?

A. Phenol coefficient test
B. Minimum inhibitory concentration test
C. Capacity test
D. None of these

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Question 32

Which of the following was the first widely used antiseptic and disinfectant?

A. Chlorine
B. Phenol
C. Iodine
D. Alcohol

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Question 33

Which of the following skin disinfectant(s) is/are most frequently used?

A. Isopropyl alcohol
B. Ethyl alcohol
C. Both A and B
D. None of these

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Question 34

Which is the most important surface active disinfectants?

A. Amphoteric compounds
B. Cationic compounds
C. Non-ionic compounds
D. Anionic compounds

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Question 35

The time required to kill 90% of the microorganisms in a sample at a specific temperature is the

A. decimal reduction time
B. thermal death point
C. F value
D. D value

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Question 36

A(n)____is used to prevent infection by killing or inhibiting pathogen growth on animal tissues.

A. bacteriostatic agent
B. sanitizer
C. disinfectant
D. antiseptic

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Question 37

Which of the following is bactericidal?

A. Membrane filtration
B. Ionizing radiation
C. Freeze-drying
D. Deep freezing

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Question 38

Bactericidal concentration of phenol is

A. 0.25%
B. 0.5%
C. 0.75%
D. 1%

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Question 39

Which of the following compounds are commonly used as general antiseptics to treat cuts and scratches?

A. Aldehydes
B. Ethylene oxide
C. Halogens
D. None of these

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Question 40

Which of the following has most heat resistant spores?

A. Clostridium histolyticum
B. Clostridium perfringens
C. Bacillus stearothermophilus
D. Bacillus cereus

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Question 41

Which of the following describes the effect of ionizing radiation on microbes?

A. Changes the 3-dimensional structure of proteins to a 2-dimen-sional form
B. Substantially decreases enzymatic activity, slowing microbial reproduction
C. Induces electrons and protons to jump out of the molecules of the microbe
D. None of these

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Question 42

Bactericidal concentration of phenol is

A. 0.25%
B. 0.50%
C. 0.75%
D. 1%

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Question 43

All of the following are sporicidal except

A. glutaraldehyde
B. ethylene oxide
C. formaldehyde
D. alcohol

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Question 44

Which of the following material is sterilized by use of red heat?

A. Soiled dressings
B. Inoculating wires and loops
C. Glass slides
D. All-glass syringes

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Question 45

Which of the following is best used for long term storage of microbial samples when carried out properly?

A. Storage in a freezer at -10°C
B. Storage in a freezer at ultra low temperatures (-70°C)
C. Storage in a refrigerator on an agar slant
D. Storage on a petri plate at room temperature

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Question 46

Which of the following describes the effect of ionizing radiation on microbes?

A. Changes the 3-dimensional structure of proteins to a 2-dimen-sional form
B. Substantially decreases enzymatic activity, slowing microbial reproduction
C. Induces electrons and protons to jump out of the molecules of the microbe
D. None of the above

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Question 47

Ethylene oxide is used to destroy or kill which of the following microbes?

A. Bacteria
B. Spores
C. Fungi
D. All of the above

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Question 48

Which of the following is not a disinfectant containing a heavy metal?

A. Silver nitrate
B. Mercurochrome
C. Copper sulfate
D. Chlorine

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Question 49

Which of the following skin disinfectant(s) is/are most frequently used?

A. Isopropyl alcohol
B. Ethyl alcohol
C. Both (a) and (b)
D. none of these

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Question 50

Which disinfectant would be most effective against Staphylococcus found in a blood spill?

A. Phenol
B. Cetylpyridinium chloride
C. Hexachlorophene
D. None of these

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Question 51

If a 1:600 dilution of a test compound kills a standard population of Staphylococcus aureus in 10 minutes but not 5 minutes while a 1:60 dilution of phenol kills the population in the same time, what is the phenol coefficient of the test compound?

A. 1
B. 5
C. 10
D. 50

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Question 52

Which of the following is sterilized with the help of glutaraldehyde?

A. Thermometers
B. Bronchoscopes
C. Cystoscopes
D. All of these

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