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Plant Cell Culture MCQ Questions & Answers

Plant Cell Culture MCQs : This section focuses on the "Plant Cell Culture". These Multiple Choice Questions (MCQs) should be practiced to improve the Plant Cell Culture skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

Organogenesis is

A. formation of callus tissue
B. formation of root and shoots on callus tissue
C. both (a) and (b)
D. genesis of organs

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Question 2

What is meant by 'Organ culture' ?

A. Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
B. Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
C. Cultivation of organs in a laboratory through the synthesis of tissues
D. The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body

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Question 3

Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?

A. Nurse medium
B. Nurse or feeder culture
C. Both A and B
D. None of the above

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Question 4

Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?

A. Multiplication of sexually derived sterile hybrids
B. Less multiplication of disease free plants
C. Storage and transportation of propogates
D. Both A and B

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Question 5

Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?

A. Multiplication of sexually derived sterile hybrids
B. Less multiplication of disease free plants
C. Storage and transportation of propogates
D. Both (b) and (c)

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Question 6

The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as

A. redifferentiation
B. dedifferentiation
C. either (a) or (b)
D. none of these

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Question 7

The ability of the component cells of callus to form a whole plant is known as

A. redifferentiation
B. dedifferentiation
C. either (a) or (b)
D. none of these

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Question 8

What is/are the benefit(s) of micropropagation or clonal propagation?

A. Rapid multiplication of superior clones
B. Multiplication of disease free plants
C. Multiplication of sexually derived sterile hybrids
D. All of the above

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Question 9

In a callus culture

A. increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
C. auxins and cytokinins are not required
D. only auxin is required for root and shoot formation

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Question 10

The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as

A. redifferentiation
B. dedifferentiation
C. Both A and B
D. None of the above

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Question 11

Protoplasts are the cells devoid of

A. cell membrane
B. cell wall
C. both cell wall and cell membrane
D. none of these

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Question 12

Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?

A. Nurse medium
B. Nurse or feeder culture
C. Both (a) and (b)
D. None of these

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Question 13

Subculturing is similar to propagation by cuttings because

A. it separates multiple microshoots and places them in a medium
B. it uses scions to produce new microshoots
C. they both use in vitro growing conditions
D. All of the above

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Question 14

Cellular totipotency is the property of

A. plants
B. animals
C. bacteria
D. all of these

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Question 15

Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?

A. Mass selection
B. Protoplast fusion
C. Transformation
D. Transpiration

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Question 16

Cell fusion method includes the preparation of large number of

A. plant cells stripped of their cell wall
B. single plant cell stripped of their cell wall
C. plant cells with cell wall
D. cells from different species

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Question 17

When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?

A. 2-5 days
B. 5-10 days
C. 10-15 days
D. 15-17 days

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Question 18

Organogenesis is

A. formation of callus tissue
B. formation of root and shoots on callus tissue
C. Both A and B
D. genesis of organs

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Question 19

Protoplasts are the cells devoid of

A. cell membrane
B. cell wall
C. both cell wall and cell membrane
D. None of the above

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Question 20

Which method of plant propagation involves the use of girdling?

A. Grafting
B. Cuttings
C. Layering
D. Micropropagation

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Question 21

A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation.

A. microshoot
B. medium
C. explant
D. scion

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Question 22

The ability of the component cells of callus to form a whole plant is known as

A. redifferentiation
B. dedifferentiation
C. Either A and B
D. None of the above

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Question 23

Agrobacterium based gene transfer is efficient

A. only with dicots
B. only with monocots
C. with both monocots and dicots
D. with majority monocots and few dicots

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Question 24

Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with

A. cellulotyic enzymes
B. pectolytic enzymes
C. both cellulotyic and pectolytic enzymes
D. proteolytic enzymes

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Question 25

Cellular totipotency is the property of

A. plants
B. animals
C. bacteria
D. All of the above

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