Plant Cell Culture MCQs : This section focuses on the "Plant Cell Culture". These Multiple Choice Questions (MCQs) should be practiced to improve the Plant Cell Culture skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Question 1
A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation.
A. microshoot
B. medium
C. explant
D. scion
Question 2
Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with
A. cellulotyic enzymes
B. pectolytic enzymes
C. both cellulotyic and pectolytic enzymes
D. proteolytic enzymes
Question 3
Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?
A. Multiplication of sexually derived sterile hybrids
B. Less multiplication of disease free plants
C. Storage and transportation of propogates
D. Both A and B
Question 4
What is meant by 'Organ culture' ?
A. Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
B. Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
C. Cultivation of organs in a laboratory through the synthesis of tissues
D. The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body
Question 5
Which method of plant propagation involves the use of girdling?
A. Grafting
B. Cuttings
C. Layering
D. Micropropagation
Question 6
Organogenesis is
A. formation of callus tissue
B. formation of root and shoots on callus tissue
C. Both A and B
D. genesis of organs
Question 7
Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?
A. Nurse medium
B. Nurse or feeder culture
C. Both A and B
D. None of the above
Question 8
In a callus culture
A. increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
C. auxins and cytokinins are not required
D. only auxin is required for root and shoot formation
Question 9
Protoplasts are the cells devoid of
A. cell membrane
B. cell wall
C. both cell wall and cell membrane
D. None of the above
Question 10
Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?
A. Mass selection
B. Protoplast fusion
C. Transformation
D. Transpiration
Question 11
The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as
A. redifferentiation
B. dedifferentiation
C. Both A and B
D. None of the above
Question 12
Cell fusion method includes the preparation of large number of
A. plant cells stripped of their cell wall
B. single plant cell stripped of their cell wall
C. plant cells with cell wall
D. cells from different species
Question 13
Subculturing is similar to propagation by cuttings because
A. it separates multiple microshoots and places them in a medium
B. it uses scions to produce new microshoots
C. they both use in vitro growing conditions
D. All of the above
Question 14
The ability of the component cells of callus to form a whole plant is known as
A. redifferentiation
B. dedifferentiation
C. Either A and B
D. None of the above
Question 15
What is/are the benefit(s) of micropropagation or clonal propagation?
A. Rapid multiplication of superior clones
B. Multiplication of disease free plants
C. Multiplication of sexually derived sterile hybrids
D. All of the above
Question 16
When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?
A. 2-5 days
B. 5-10 days
C. 10-15 days
D. 15-17 days
Question 17
Cellular totipotency is the property of
A. plants
B. animals
C. bacteria
D. All of the above
Question 18
Agrobacterium based gene transfer is efficient
A. only with dicots
B. only with monocots
C. with both monocots and dicots
D. with majority monocots and few dicots
Question 19
Cellular totipotency is the property of
A. plants
B. animals
C. bacteria
D. all of these
Question 20
Organogenesis is
A. formation of callus tissue
B. formation of root and shoots on callus tissue
C. both (a) and (b)
D. genesis of organs
Question 21
Protoplasts are the cells devoid of
A. cell membrane
B. cell wall
C. both cell wall and cell membrane
D. none of these
Question 22
The ability of the component cells of callus to form a whole plant is known as
A. redifferentiation
B. dedifferentiation
C. either (a) or (b)
D. none of these
Question 23
The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as
A. redifferentiation
B. dedifferentiation
C. either (a) or (b)
D. none of these
Question 24
Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?
A. Multiplication of sexually derived sterile hybrids
B. Less multiplication of disease free plants
C. Storage and transportation of propogates
D. Both (b) and (c)
Question 25
Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?
A. Nurse medium
B. Nurse or feeder culture
C. Both (a) and (b)
D. None of these