Protein and Nucleic Acid Interactions MCQs : This section focuses on the "Protein and Nucleic Acid Interactions". These Multiple Choice Questions (MCQs) should be practiced to improve the Protein and Nucleic Acid Interactions skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?
A. Asn and Gln
B. Cys and Met
C. Lys and Leu
D. Ile and Val
Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
A. positive direction
B. 3' to 5' direction
C. 5' to 3' direction
D. negative direction
Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?
A. Ile and Val
B. Lys and Arg
C. Leu and Ala
D. Cys and Met
Proteins interact with DNA and RNA through similar physical forces, which include electrostatic interactions (salt bridges), dipolar interactions (hydrogen bonding, H-bonds), entropic effects (hydrophobic interactions) and dispersion forces (base stacking).
The enzyme that joins DNA cuts is called
C. DNA phosphorylase
D. reverse transcriptase
specific protein–DNA interactions are commonly mediated by an α-helix motif in the protein that inserts itself into the major groove of the DNA, recognizing and interacting with a specific base sequence through H-bonds and salt bridges.
Reverse transcripase from HIV does not
A. uses RNA as a template
B. has a 5' to 3' polymerase activity
C. has a 3'- 5' exonuclease activity
D. requires a primer
The DNA- or RNA-binding function of a protein is localized in discrete conserved domains within its tertiary structure.
Cleavage of DNA by EcoRI endonuclease results in the formation of
A. two 3'-OH ends and two 5'-phosphate ends
B. one 3'-OH end and one 5'-phosphate end
C. two 3'-phosphate ends and two 3'-OH ends
D. one 3'-OH end and two 5'-phosphate end
The type II restriction endonucleases, bind to DNA sites
A. using H-bonds for specificity
B. that are usually palindromic
C. using non-sequence-dependent backbone interactions
D. all of the above