Recombinant DNA MCQ Questions & Answers

Question 1

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

A. southern blotting
B. northern blotting
C. polymerase chain reaction
D. DNA replication

Question 2

The Southern blotting technique depends on

A. similarities between the sequences of probe DNA and experimental DNA
B. similarities between the sequences of probe RNA and experimental RNA
C. similarities between the sequences of probe protein and experimental protein
D. the molecular mass of proteins

Question 3

Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

A. BACS
B. expression vectors
C. YACS
D. All of the above

Question 4

Agrobacterium tumefaciens is

A. a disease in humans that causes loss of sight
B. a bacterium that can be used to introduce DNA into plants
C. a fungi that is used to produce antibiotics in large amounts
D. a disease in humans that causes loss of weight

Question 5

In genetic engineering, a chimera is

A. an enzyme that links DNA molecules
B. a plasmid that contains foreign DNA
C. a virus that infects bacteria
D. a fungi

Question 6

The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

A. genetic engineering
B. population genetics
C. microbiology
D. protein engineering

Question 7

Electroporation is

A. the process of separating charged molecules through a gel maintained in an electric field
B. the process of combining foreign DNA to an electrically charged vector molecule
C. the application of high voltage pulses
D. the process of multiplication of the cells

Question 8

Which of the following is obtained using processed mRNA molecules as a template?

A. rDNA
B. mDNA
C. cDNA
D. tDNA

Question 9

Vectors are

A. molecules that degrade nucleic acids
B. molecules that help in replication
C. molecules that are able to covalently bond to and carry foreign DNA into cells
D. molecules that protect host cells from invasion by foreign DNA

Question 10

What is the normal role of restriction endonucleases in bacterial cells?

A. To degrade the bacterial chromosome into small pieces during replication
B. To degrade invading phage DNA
C. To produce RNA primers for replication
D. All of the above

Question 11

Which of the following is not commonly used as vector?

A. Artificial chromosome
B. Cosmid
C. Fungi
D. Plasmid

Question 12

A short molecule containing 2-20 nucleotide is

A. plasmid
B. vector
C. oligonucleotide
D. mononucleotide

Question 13

Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

A. DNA ligase
B. helicases
C. restriction endonucleases
D. DNA gyrase

Question 14

Which of the following vector can maintain the largest fragment of foreign DNA?

A. YAC
B. Cosmid
C. Plasmid
D. Phage

Question 15

Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

A. photoreactivation
B. gel electrophoresis
C. autoradiography
D. blotting

Question 16

For gene probes to be useful they must

A. be large enough to contain gene-specific sequences
B. be labeled in some manner to allow detection
C. Both A and B
D. None of these

Question 17

A genomic library is

A. a database where the sequence of an organism's genome is stored
B. a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
C. a book that describes how to isolate DNA from a particular organism
D. a place where the information of the genetic organization of organisms are kept.

Question 18

Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

A. DNA polymerase
B. Restriction endonuclease
C. DNA ligase
D. DNA helicase

Question 19

Bacterial cells protect their own DNA from degradation by restriction endonucleases by

A. methylating the DNA at the sites that the enzyme recognizes
B. deleting all recognition sites from the genome
C. not producing any restriction endonucleases
D. having anti restriction endonucleases

Question 20

Which type of restriction endonuclease cuts the DNA within the recognition site?

A. Type I
B. Type II
C. Type III
D. All of the above

Question 21

An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

A. a chimera
B. a transgenic animal
C. a vector
D. an enzyme that links DNA molecules

Question 22

For gene probes to be useful they must

A. be large enough to contain gene-specific sequences
B. be labeled in some manner to allow detection
C. both (a) and (b)
D. none of the above

Question 23

Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

A. BACS
B. expression vectors
C. YACS
D. all of these

Question 24

The advantage of using DNA polymerases from thermophilic organisms in PCR is that

A. the DNA polymerases of these bacteria are much faster than those from other organisms
B. the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
C. the DNA polymerases of these bacteria never make mistakes while replicating DNA
D. all of the above

Question 25

The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

A. laser
B. DNA probe
C. gene gun
D. inoculating needle

Question 26

Which type of restriction endonuclease cuts the DNA within the recognition site?

A. Type I
B. Type II
C. Type III
D. All of the these