Recombinant DNA Technology MCQs : This section focuses on the "Recombinant DNA Technology". These Multiple Choice Questions (MCQs) should be practiced to improve the Recombinant DNA Technology skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Question 1
An expression vector
A. always contains an origin of replication
B. usually contains a gene that confers antibiotic resistance to the bacterial host
C. always contains DNA segments for the regulation of mRNA production
D. all of the above
Question 2
Which of these restriction enzymes produce blunt ends?
A. SaII
B. EcoRV
C. XhoI
D. HindIII
Question 3
Which of the following is not true of small inbreeding populations?
A. They tend to loose genetic diversity
B. There is an increased incidence of recessive diseases
C. Alleles may become fixed
D. Mutation is increased
Question 4
Which of the following pair will produce complementary sticky ends?
A. Eco RI & MspI
B. Msp I & HPA II
C. Sau 3A & Bam HI
D. Mbo I & Sau 3A
Question 5
Which of the following techniques can be used to determine the defective gene and for developing cancer?
A. Western blot
B. Southern blot
C. Northern blot
D. Eastern blot
Question 6
Restriction enzymes
A. protect bacteria from viral infection
B. cut DNA in a staggered fashion
C. cut DNAs producing a blunt end
D. all of the above
Question 7
Which of the following genetic diseases would be amenable to genetic engineering?
A. Down's syndrome
B. Muscular dystrophy
C. Cystic fibrosis
D. Cri du Chat
Question 8
The unpaired nucleotides produced by the action of restriction enzymes are referred to have
A. sticky ends
B. single strands
C. restriction fragments
D. ligases
Question 9
What enzyme forms covalent bonds between restriction fragments?
A. DNA primase
B. DNA helicase
C. DNA polymerase
D. DNA ligase
Question 10
Plant transformation can be obtained by
A. combining plant and animal cells in culture
B. shooting DNA into plant cells with a gun
C. using the E. coli bacterium to infect plant roots
D. infecting plants with a tobacco mosaic virus
Question 11
When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .
A. assortative mating
B. inbreeding
C. heterosis
D. genetic drift
Question 12
In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel.
A. negative ... positive
B. basic ... acidic
C. long ... short
D. positive to negative
Question 13
Knockout mice are created by
A. mutagenizing a mouse and selecting for mutant offspring
B. creating a chimera by fusing cells from two different cell lines
C. infecting the mouse with a retrovirus
D. transfecting embryonic stem cells with an altered gene sequence
Question 14
The TP53 gene of chromosome 17 codes for a protein __________ .
A. that plays a role in the digestive process
B. involved in glucose transport
C. involved in the regulation of the cell cycle
D. that is like a white blood cell protein
Question 15
Which type of restriction enzymes do not usually require ATP?
A. Type I
B. Type II
C. Type III
D. Type IV
Question 16
A plasmid
A. is a circular DNA molecule
B. always contains an origin of replication
C. usually contains one or more restriction sites
D. all of the above
Question 17
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are
A. copies of the gene with an altered sequence so that a restriction site is inserted
B. mutagenized copies of a gene
C. short sequences that will hybridize only to a specific base sequence
D. PCR-amplified variable numbers of tandem repeats (VNTRs)
Question 18
An example of a restriction fragment length polymorphism is
A. an Eco RI cuts DNA at a different sequence than Hind III
B. different length fragments of DNA resulting from loss or gain of a restriction site
C. cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
D. all of the above
Question 19
The order for the construction of a cDNA fragment from mRNA is to
A. bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
B. treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
C. digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
D. bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
Question 20
Which of these genes codes for a protein that plays a role in growth?
A. DCP1
B. SCLC6A4
C. KRTHA1
D. GH1
Question 21
Under which of the following conditions would population gene frequencies remain the same?
A. Selection for homozygotes
B. Small population size
C. Active migration between groups
D. Random mating
Question 22
Which of these genes codes for a protein that plays a role in white blood cell function?
A. DCP1
B. MPO
C. GLUT4
D. RP13
Question 23
Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because
A. in both cases molecules migrate to the anode
B. both techniques rely on a constant charge to mass ratio
C. both techniques utilize the sieving properties of gels
D. all of the above
Question 24
Which of the following can be diagnosed by amniocentesis?
A. Down's syndrome
B. Cystic Fibrosis
C. Sickle cell anemia
D. All of these
Question 25
First discovered, Type II restriction endonuclease was
A. Hinf I
B. Eco K
C. Hind II
D. EcoRI
Question 26
The RP13 gene of chromosome 17 codes for a protein __________ .
A. involved in glucose transport
B. that is a component of hair and nails
C. involved in eye development
D. involved in the determination of personality
Question 27
Why is golden rice pale yellow in color?
A. It is rich in chlorophyll a.
B. It is rich in beta-carotene.
C. It is rich in chlorophyll b.
D. It is rich in phycobilins.
Question 28
Which of the following is correct in terms of determination of location of genetic traits?
A. Known protein coding sequences are too far apart to allow linkage determination for most new genes
B. Restriction sites allow DNAs to be digested
C. Protein-coding genes are always associated with a restriction pattern
D. None of the above
Question 29
The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .
A. would be of no concern if it occurred.
B. has occurred
C. can never occur
D. seems unlikely
Question 30
In order to insert a foreign gene into a plasmid, both must __________
A. have identical DNA sequences
B. originate from the same type of cell
C. be cut by the same restriction enzyme
D. be of the same length
Question 31
Isoschizomers recognize
A. same recognition sequence but different recognition site
B. same recognition site and recognition sequence
C. same recognition site and different recognition sequence
D. different recognition site and different recognition sequence
Question 32
Restriction enzymes are named for
A. the person who discovered
B. the bacterium they are derived from
C. the viral DNA that they attack
D. none of the above
Question 33
A human cDNA library
A. contains DNA for specific human proteins
B. contains DNA for virtually all of the human proteins in vectors
C. cannot be used to obtain human genes because it would be radioactive
D. none of the above
Question 34
X-rays cause
A. the formation of thymine dimers
B. ionization of water in the cell
C. heat
D. none of the above