DAPZOI
Home Ask MCQ Quiz Topics Login


Transcription and Regulation MCQ Questions & Answers

Transcription and Regulation MCQs : This section focuses on the "Transcription and Regulation". These Multiple Choice Questions (MCQs) should be practiced to improve the Transcription and Regulation skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

Which of the following is necessary for transcription to occur?

A. DNA molecule
B. RNA polymerase
C. DNA polymerase
D. Both DNA & RNA polymerase

View Answer

Question 2

In prokaryotes, transcription is

A. terminated by the stop codon
B. terminated by a protein called rho
C. terminated by a poly A sequence
D. terminated by a start codon

View Answer

Question 3

The function of the sigma factor of RNA polymerase is to

A. assure that transcription begins at the proper point
B. assure that transcription ends at the proper point
C. assure that translation begins at the proper point
D. assure that translation ends at the proper point

View Answer

Question 4

The transcriptional regulatory proteins in eukaryotes which induce transcription usually have

A. at least two distinct domains of protein structure, a DNA binding domain and an activation domain
B. at least two distinct domains of protein structure and a DNA binding domain
C. at least one distinct domains of protein structure, a DNA binding domain and an activation domain
D. none of these

View Answer

Question 5

What is a promoter?

A. A specific sequence of DNA to which a catabolic repressor binds
B. A specific sequence of DNA to which RNA polymerase binds
C. A specific DNA sequence to which a restriction endonuclease binds
D. None of the above

View Answer

Question 6

The complex of RNA polymerase, DNA template and new RNA transcript is called

A. transcription bubble
B. replication bubble
C. a translation bubble
D. none of these

View Answer

Question 7

In both eukaryotes and prokaryotes, a promoter region that is rich in AT bases is called as

A. CATT
B. Shine Dalgarno region
C. TATA box
D. SV40 region

View Answer

Question 8

In eukaryotes, there are three different RNA polymerases. The RNA polymerase responsible for transcription of mRNA is

A. RNA polymerase I
B. RNA polymerase II
C. RNA polymerase III
D. none of these

View Answer

Question 9

Transcription of each set of rRNA genes by RNA polymerasel produces

A. a single 45S rRNA precursor molecule
B. a single 5S rRNA precursor molecule
C. a single 18S rRNA precursor molecule
D. a single 28S rRNA precursor molecule

View Answer

Question 10

Enhancers are regions that

A. bind RNA polymerase
B. are adjacent to the TATA box
C. are CAT box binding proteins
D. modulate transcription

View Answer

Question 11

The binding of lac repressor to DNA could be considered to be analogous to

A. competitive inhibition of an enzyme
B. mixed-type inhibition of an enzyme
C. uncompetitive inhibition of an enzyme
D. allosteric effects in enzyme regulation

View Answer

Question 12

RNA polymerase in prokaryotes has a removable

A. alpha subunit
B. beta subunit
C. both (a) and (b)
D. sigma subunit

View Answer

Question 13

Transcription factors are

A. promoters
B. proteins which bind to DNA and regulate transcription
C. TATA boxes
D. CATT boxes

View Answer

Question 14

Promoters for tRNAs are located

A. upstream from the start codon
B. downstream from the start codon
C. both (a) and (b)
D. none of these

View Answer

Question 15

Most transcripts of protein coding genes are cleaved

A. at the 3' end just after a poly adenylation signal
B. at the 3' end just before a poly adenylation signal
C. at the 5' end immediately after a poly adenylation signal
D. at the 5' end immediately before a poly adenylation signal

View Answer

Question 16

The nucleons of eukaryotic cell has

A. RNA polymerase I
B. RNA polymerase II
C. RNA polymerase III
D. all of these

View Answer

Question 17

The closed complex at bacterial promoters is

A. in equilibrium with free RNA polymerase and the promoter
B. not affected by promoter mutations
C. bound more tightly when repressors are present
D. not affected by activator proteins

View Answer

Question 18

RNA is synthesized on a DNA template in a process called __________ , which utilizes the enzyme __________

A. translation, RNA polymerase
B. transcription, DNA polymerase
C. transcription, RNA polymerase
D. replication, DNA polymerase

View Answer

Question 19

The regions of a gene located at -10 and -35, with respect to the start of transcription, are termed

A. start codons.
B. oriC regions.
C. Shine Dalgarno sequences.
D. promoters.

View Answer

Question 20

The RNA polymerases that transcribe bacterial DNA are

A. multisubunit enzymes
B. only active inside the cell
C. interchangable with DNA polymerases
D. highly glycosylated in their active forms

View Answer

Question 21

Transcription begins

A. at the start codon
B. upstream from the start codon
C. downstream from the start codon
D. none of these

View Answer

Question 22

Immediately after transcription

A. a methylated guanine cap is added to the 51 end of the transcript
B. a methylated guanine cap is added to the 31 end of the transcript
C. both (a) and (b)
D. a poly adenylation signal is added

View Answer

Question 23

Rho-dependent termination of transcription in E. coli

A. requires ATP
B. requires about 50 nucleotides of uncomplexed mRNA
C. both (a) and (b)
D. removes mRNA and holoenzyme from the DNA

View Answer

Question 24

Multiple copies of 5S genes, located at a chromosomal site distinct from the other rRNA genes

A. are transcribed by rRNA polymerase III and the 5S rRNA
B. are transcribed by rRNA polymerase II
C. are transcribed by rRNA polymerase I and the 5S rRNA
D. are transcribed by DNA polymerase I

View Answer

Question 25

The major function of RNA polymerase's sigma factor is

A. recognition of the translational stop sequence
B. recognition of the transcriptional start sequence
C. recognition of the transcriptional stop sequence
D. recognition of the translational start sequence

View Answer

Question 26

Transcription results in

A. an amino acid chain
B. messenger RNA
C. complementary DNA
D. Okazaki fragments

View Answer

Question 27

Each tRNA molecule has a cloverleaf secondary structure consisting of

A. three stem loops
B. two stem loops
C. four stem loops
D. either (a) or (b)

View Answer

Question 28

Which of the following conditions would cause the release of the lac repressor protein from the lac operator site on DNA?

A. Presence of glucose in the growth media
B. Presence of lactose in the growth media
C. Presence of IPTG (isopropyl thiogalactoside) in the growth media
D. Both (b) and (c)

View Answer

Question 29

Transcription by E. coli polymerase occurs in

A. four phases known as initiation, propagation, elongation and termination
B. three phases known as initiation, elongation and termination
C. two phases known as initiation and termination
D. none of the above

View Answer

Question 30

RNA polymerase holoenzyme initiates transcription which involves

A. recognition of the -10 sequence and -35 sequence
B. recognition of the -10 sequence only
C. recognition of the -35 sequence only
D. none of the above

View Answer

Question 31

Upstream regions which are essential for correct transcription are called

A. enhancers
B. start codons
C. promoters
D. transcription factors

View Answer