Transcription MCQs : This section focuses on the "Transcription". These Multiple Choice Questions (MCQs) should be practiced to improve the Transcription skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Question 1
Which of the following enzyme is used for synthesis of RNA under the direction of DNA?
A. RNA polymerase
B. DNA ligase
C. DNA polymerase
D. RNA ligase
Question 2
What is the usual sequence of a Pribnow box?
A. AUAUA
B. TATAAT
C. UUUUU
D. TTGACA
Question 3
Which of the following component of RNA polymerase facilitates the recognition of promoter sequences?
A. a subunit
B. b subunit
C. s subunit
D. c subunit
Question 4
Which of the following statement defines a replicon?
A. A DNA molecule that encodes pili for conjugation
B. A DNA molecule that is able to replicate and be maintained
C. A DNA template which is used in transcription
D. The enzyme responsible for transposition
Question 5
Which eukaryotic RNA polymerase makes tRNA's?
A. RNA polymerase 1
B. RNA polymerase 2
C. RNA polymerase 3
D. Any of the above
Question 6
The location of first hexameric sequence typically found in pro-karyotic promoters is
A. at the transcription start site
B. approximately 35 bases upstream of the transcription start site
C. approximately 10 bases upstream of the transcription start site
D. approximately 25 bases upstream of the transcription start site
Question 7
Which of the following is a product of transcription?
A. mRNA
B. tRNA
C. rRNA
D. All of the above
Question 8
The sigma factor
A. is responsible for recognizing the termination sequence
B. provides the catalytic function of cleaving off pyrophosphates during mRNA synthesis
C. recognizes the promotor
D. accomplishes DNA restriction
Question 9
The synthesis of mRNA based on a DNA template is called
A. DNA replication
B. transcription
C. translation
D. DNA restriction
Question 10
Recognition/binding site of RNA polymerase is called
A. receptor
B. promoter
C. facilitator
D. terminator
Question 11
Which of the following enzyme(s) is/are required for lactose fermentation?
A. Transacetylase
B. β-galactosidase
C. Galactoside permease
D. All of the above
Question 12
Which of these catalyzes the synthesis of RNA from a DNA template?
A. DNA gyrase
B. DNA ligase
C. DNA polymerase
D. RNA polymerase
Question 13
A level of regulation, demonstrated by the termination of transcription if tryptophan is abundant, by the tryptophan operon in E.coli cells is called
A. activation
B. attenuation
C. corepression
D. desiccation
Question 14
An mRNA transcript of a gene contains
A. a start codon
B. a stop codon
C. a terminator
D. All of the above
Question 15
Which of the following proteins first binds to TATA box during transcription?
A. TF II A
B. TF II D
C. TF II B
D. RNA polymerase
Question 16
The components found in all prokaryotic transcription terminators is
A. a poly-U region
B. Rho factor
C. a hairpin structure
D. None of these
Question 17
A specific __________ factor helps RNA polymerase bind to late promoters and transcribe late genes.
A. sigma
B. beta
C. delta
D. gamma
Question 18
An mRNA transcript of a gene contains
A. a start codon
B. a stop codon
C. a terminator
D. all of these
Question 19
Which eukaryotic RNA polymerase makes tRNA's?
A. RNA polymerase 1
B. RNA polymerase 2
C. RNA polymerase 3
D. Any of these
Question 20
Which of the following enzyme(s) is/are required for lactose fermentation?
A. Transacetylase
B. β-galactosidase
C. Galactoside permease
D. All of these
Question 21
Which of the following is a product of transcription?
A. mRNA
B. tRNA
C. rRNA
D. all of these
Question 22
After transcribing into RNA, the gene is spliced out of the genome.
A. True
B. False
Question 23
In bacteria, binding of _________ factor to the RNA polymerase increases its affinity for promoter sites in the DNA.
A. alpha
B. sigma
C. rho
D. beta
Question 24
In patients of sickle cell anemia, the hemoglobin contains a substitution for ____________________
A. glutamic acid
B. ascorbic acid
C. carboxylic acid
D. quinone
Question 25
Promoter is a ____________________
A. gene
B. site on DNA
C. site on RNA
D. toxin
Question 26
RNA is a mobile nucleic acid.
A. True
B. False
Question 27
The transcription factors assist the RNA polymerase in locating ________________
A. stop codon
B. mutation
C. active site
D. promoter
Question 28
Transcription is the synthesis of ______________________
A. RNA
B. DNA
C. Protein
D. Polypeptide
Question 29
What is the intermediate between a gene and its polypeptide?
A. mRNA
B. tRNA
C. rRNA
D. siRNA
Question 30
When was the link between enzymes and inherited disease first elucidated?
A. 1708
B. 1808
C. 1908
D. 2008
Question 31
Which enzyme makes the transcription, an essentially irreversible process?
A. pyrophosphatase
B. amylase
C. glucamylase
D. topoisomerase
Question 32
Which of the following enzymes are used in the process of transcription?
A. DNA polymerases
B. RNA polymerases
C. DNA helicase
D. DNA topoisomerase
Question 33
Which of the following play a role in structural support?
A. rRNA
B. mRNA
C. tDNA
D. tRNA
Question 34
Which of the two strands of DNA will be transcribed is determined by the _______________
A. RNA polymerase
B. DNA polymerase
C. Promoter sequence
D. Inducer sequence
Question 35
Which organism was used for experiments that led to “one gene – one enzyme” hypothesis?
A. Neurospora
B. Nematode
C. Mus musculus
D. Drosophila
Question 36
Which proteolytic enzyme was used in the experiments aimed at elucidating the mutation that causes sickle cell anemia?
A. papain
B. rennin
C. pepsin
D. trypsin
Question 37
How many base pairs of DNA is transcribed by RNA polymerase in one go?
A. 5-6
B. 3
C. 4
D. 7-8
Question 38
Like replication, transcription also occurs bidirectionally.
A. True
B. False
Question 39
Mark the one, which is NOT the transcription inhibitor in eukaryotes.
A. Rifampicin
B. Acridine dye
C. Actinomycin D
D. Rho factor
Question 40
Mark the statement which is INCORRECT about the transcription unit?
A. It is a transcribed segment of DNA
B. Eukaryotes have monocistronic transcription unit
C. Prokaryotes also have a monocistronic transcription unit
D. Immediate product of transcription is primary transcript
Question 41
Name the one intrinsic terminator of transcription.
A. Intercalating agents
B. Rho independent
C. Rho dependent
D. Acridine orange
Question 42
Name the sigma factor which is used for promoter recognition?
A. Sigma 32
B. Sigma 70
C. Sigma 60
D. Sigma 40
Question 43
Name the site where upstream sequences located?
A. Prior to start point
B. After the startpoint
C. Right border of DNA
D. In the middle of DNA
Question 44
The process of formation of RNA is known as___________
A. Replication
B. DNA repair
C. Translation
D. Transcription
Question 45
What is the work of the sigma factor in transcription?
A. Helicase action
B. Transcription initiation
C. Transcription elongation
D. Transcription termination
Question 46
Which of the following ensure stable binding of RNA polymerase at the promoter site?
A. DNA photolyase
B. Sigma factor
C. DNA glycosylase
D. RecA
Question 47
Which of the following is TRUE for the RNA polymerase activity?
A. DNA dependent DNA synthesis
B. Direct repair
C. DNA dependent RNA synthesis
D. RNA dependent RNA synthesis
Question 48
Which of the following is used to describe the time taken by RNA polymerase to leave the promoter?
A. Promoter clearance time
B. Abortive initiation
C. Elongation factor
D. Mean time
Question 49
Which of the following transcription termination technique has RNA dependent ATPase activity?
A. Intercalating agents
B. Rho dependent
C. Rho independent
D. Rifampcin