Transgenic Plant MCQs : This section focuses on the "Transgenic Plant". These Multiple Choice Questions (MCQs) should be practiced to improve the Transgenic Plant skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Question 1
In a __________ protocol, bacteria with engineered abilities to detoxify pollutants are intentionally released in an area.
A. microcosm establishment
B. mibridization
C. bioremediation
D. rhizosecretion
Question 2
The controversy regarding the use of Bt corn is that it
A. is potentially harmful to monarch butterflies
B. is a potential allergen to humans
C. Both A and B
D. can contaminate groundwater
Question 3
Metabolic interference is a term used to describe a method to metabolize a compound and prevent the synthesis of something that is normally produced. What compound(s) have been targeted for metabolic interference in tomato?
A. ACC (1-aminocyclopropane-1-carboxylic acid)
B. SAM (S-adenosylmethionine)
C. Both A and B
D. AOA (aminooxyacetic acid)
Question 4
Starch content of potatoes can be increased by using a bacterial gene, known as
A. sucrose phosphate synthase gene
B. ADP glucose pyrophosphorylase gene
C. polygalactouranase gene
D. None of the above
Question 5
Which tropical fruit crop has been successfully engineered to be protected against a lethal virus?
A. Passion fruit
B. Papaya
C. Mango
D. Lychee
Question 6
Which of the following metabolites are implicated in stress tolerance?
A. Proline
B. Betaines
C. Both A and B
D. Citrate
Question 7
Which of the agricultural challenges below cannot be solved with transgenic techniques?
A. Crops are damaged by frost
B. Crops are killed by a virus
C. Public concern about safety of synthetic pesticides
D. Public preference for organic vegetables
Question 8
Which of the following statements is true regarding genomics?
A. Plant genomics lags behind similar efforts in animals and microorganisms
B. Researchers can make use of genomic information even if the entire genome of an organism is not known
C. Researchers are busy in trying to determine the genome of the potato plant
D. All of the above
Question 9
Which of the following gene is responsible for resistance against chilling?
A. Glycerol 1 phosphate acyl transferase
B. Polygalactouranase
C. ACC deaminase
D. Sucrose phosphate synthase gene
Question 10
Which of the following gene detoxifies herbicide phosphinothricin?
A. Nitrilase
B. Glutathione S-transferase (GST)
C. Phosphinothricin acetyl transferase
D. All of the above
Question 11
Plants derived sexually from the same plant are____ while those derived from somatic tissue from the same plant are____.
A. identical, different
B. different, also different
C. different, identical
D. plants cannot be derived from somatic tissue
Question 12
Transgenic plants with increased tolerance to aluminum have been produced by making plants that
A. secrete phytosiderophores into the soil
B. make more metal-binding peptides like phytochelatins
C. bind aluminum to the cell wall
D. secrete citrate into the soil
Question 13
Transplastomics
A. targets genes in the chloroplast
B. provides exceptionally low yields of protein products
C. produces genes that are released in pollen
D. offers little opportunity for practical use
Question 14
Plants containing genes encoding cytokines and blood clotting factors are used in
A. nutrition improvement
B. pharmaceutical production
C. vaccine production
D. textile production
Question 15
The first transgenic plants expressing engineered foreign genes were tobacco plants produced by the use of
A. Agrobacterium tumefaciens
B. Bacillus thuringiensis
C. Arabidopsis thaliana
D. Streptomyces hygroscopicus
Question 16
Transgenic plants
A. contain foreign genes in their cells
B. are used to produce human antibodies
C. Both A and B
D. are plants that differ in geographical locations
Question 17
Low temperatures induce the expression of many cold-induced genes. Transgenic plants with improved cold tolerance have been produced by
A. expressing the protein that activates expression of these genes all the time in plants
B. cooling plants using the Peltier effect
C. expressing a gene for production of antifreeze (ethylene glycol) in plants
D. increasing evaporative cooling from leaf surfaces
Question 18
Nitrilase is encoded by
A. gene bxn in Klebsiella pneumoniae
B. bar gene in Streptomyces spp
C. Both A and B
D. None of the above
Question 19
If the goal were to create a plant resistant to an insecticide, which cell-based plant technology would be most effective?
A. Clonal propagation
B. Cybridization
C. Protoplast fusion
D. Mutant selection
Question 20
What are the various disadvantages of cross protection?
A. Possibility of mutations in inducing mild virus strain
B. Possibility of synergism between inducing virus and other unrelated virusPossibility of synergism between inducing virus and other unrelated virus
C. Possibility of unnecessary spread of mild virus
D. All of the above
Question 21
A __________ is a small laboratory set up to simulate conditions of a particular environment.
A. microcosm
B. mibridization
C. bioremediation
D. rhizosecretion
Question 22
A naturally occurring variant, possessing characteristics of interest, is identified. This plant is selectively bred. This is an example of
A. traditional plant breeding
B. transgenic technology
C. mutant selection
D. none of these
Question 23
ACC demainase gene is responsible for
A. synthesis for ethylene
B. degradation of ACC an immediate precursor to ethylene
C. both (a) and (b)
D. synthesis of polygalactouranase
Question 24
Antisense technology
A. selectively blocks expression of a gene
B. combines genetic material from different species
C. combines organelles and cells
D. alters or transfers cells
Question 25
Antisense transgenic plants produced fruit that softened
A. more slowly than the normal fruit
B. more rapidly than the normal fruit
C. as the normal fruits
D. none of these
Question 26
Bruise resistant tomatoes have been developed by the expression of antisense RNA against
A. glycerol 1 phosphate acyl transferase
B. polygalactouranase
C. ACC deaminase
D. Sucrose phosphate synthase gene
Question 27
Cross protection against viruses in transgenic plants can be obtained by
A. inoculating the susceptible strain of a crop with a mild strain of a virus that helps in developing resistance against more virulent strain
B. inoculating the susceptible strain with the coat proteins of virulent strain
C. inoculating the susceptible strain with genes of nucleocapsid
D. any of the above
Question 28
Expression of antisense RNA in transgenic plants is a general method used to
A. activate the expression of all genes in a biochemical pathway
B. eliminate the expression of all genes in a biochemical pathway
C. block the expression of virus coat protein genes
D. reduce or eliminate the expression of individual genes
Question 29
For developing herbicide resistance in transgenic plants which of the following approach/(es) is/are used?
A. Target molecule is made insensitive to herbicide
B. Target protein is overproduced
C. A pathway should be introduced that detoxify the herbicide
D. All of the above
Question 30
Given the DNA sequence ATTAAGGC, which sequence would be used by researchers as an antisense molecule?
A. AUUAAGGC
B. UAAUUCCG
C. CGGAATTA
D. TAATTCCG
Question 31
In transgenic plants that have been manipulated to express patho-genesis-related (PR) proteins all the time it has been shown that
A. these provide no protection at all against pathogens
B. expression of one PR protein provides high levels of protection against many pathogens
C. expression of two PR proteins provides some protection against a few pathogens
D. these proteins trigger the production of other antimicrobial defenses
Question 32
Insect resistance in the transgenic plant has been achieved by
A. transferring genes for Bt toxins
B. transferring genes for protease inhibitors
C. transferring genes for other insecticidal secondary metabolities
D. all of the above
Question 33
Metabolic interference is a term used to describe a method to metabolize a compound and prevent the synthesis of something that is normally produced. What compound(s) have been targeted for metabolic interference in tomato?
A. ACC (1-aminocyclopropane-1-carboxylic acid)
B. SAM (S-adenosylmethionine)
C. Both (a) and (b)
D. AOA (aminooxyacetic acid)
Question 34
Nitrilase is encoded by
A. gene bxn in Klebsiella pneumoniae
B. bar gene in Streptomyces spp
C. both (a) and (b)
D. none of these
Question 35
Phosphinothricin acetyl transferase is encoded by
A. gene bxn in Klebsiella pneumoniae
B. bar gene in Streptomyces spp
C. both (a) and (b)
D. none of these
Question 36
Reducing the expression of pectin methylesterase (PME) has resulted in
A. plants with resistance to bacteria and fungi
B. tomato juice that has higher viscosity
C. tomato fruit that last longer before they become rotten
D. tomato fruit that will not ripen
Question 37
Resistance to glyphosphate in transgenic petunia plants has been developed by the transfer of
A. gene for EPSPS(5-enol-pyruvyl shikimat 3 phosphate synthase)
B. gene for ALS (acetolactate synthase)
C. gene for GS (glutamine synthase)
D. any of the above
Question 38
Resistance to L-phosphinothrium in tobacco plants is developed by the transfer of
A. gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase)
B. gene for ALS (acetolactate synthase)
C. gene for GS (glutamine synthase)
D. any of the above
Question 39
Resistance to sulphonyl urea compounds in transgenic tobacco plant has been developed by the transfer of
A. gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase)
B. gene for ALS (acetolactate synthase)
C. gene for GS (glutamine synthase)
D. any of the above
Question 40
The controversy regarding the use of Bt corn is that it
A. is potentially harmful to monarch butterflies
B. is a potential allergen to humans
C. both (a) and (b)
D. can contaminate groundwater
Question 41
The delayed ripening tomato was created by a biotechnologist who __________ a gene.
A. altered
B. silenced
C. replaced
D. relocated
Question 42
The first field tests were conducted with which of the following genetically altered organism?
A. bt Corn
B. Vaccinia virus containing a gene from the rabies virus
C. The flavrsavr tomato
D. Strawberry seedlings sprayed with ice-minus bacteria
Question 43
The roots of a plant are converted into drug-producing structures in a process called
A. microcosm establishment
B. mibridization
C. bioremediation
D. rhizosecretion
Question 44
Tomatoes exhibiting delayed ripening express antisense RNA against
A. glycerol 1 phosphate acyl transferase
B. polygalactouranase
C. ACC deaminase
D. sucrose phosphate synthase gene
Question 45
Transgenic plants
A. contain foreign genes in their cells
B. are used to produce human antibodies
C. both (a) and (b)
D. are plants that differ in geographical locations
Question 46
Two genes from a bacterium (Alcaligenes eutrophus) and a cotton gene produce
A. a plastic
B. a vaccine against the bacterium
C. a pharmaceutical product
D. herbicide resistance
Question 47
What are the various disadvantages of cross protection?
A. Possibility of mutations in inducing mild virus strain
B. Possibility of synergism between inducing virus and other unrelated virus
C. Possibility of unnecessary spread of mild virus
D. All of the above
Question 48
Which cell-based plant technology involves the combining of two cells without cell walls from different species?
A. Clonal propagation
B. Cybridization
C. Protoplast fusion
D. Mutant selection
Question 49
Which cell-based technology endows a cell with increased ability to harness energy?
A. Chlybridization
B. Cybridization
C. Mibridization
D. Protoplast fusion
Question 50
Which of the following compounds has been produced in transgenic plants to improve tolerance to salt stress and water deficit?
A. Sucrose
B. Mannitol
C. Nicotine
D. Octopine
Question 51
Which of the following dies from Ti plasmid infection?
A. Rice
B. Corn
C. Sorghum
D. All of these
Question 52
Which of the following gene detoxifies herbicide phosphinothricin?
A. Nitrilase
B. Glutathione S-transferase (GST)
C. Phosphinothricin acetyl transferase
D. All of these
Question 53
Which of the following gene detoxify herbicide bronoxynil?
A. Nitrilase
B. Glutathione S-transferase (GST)
C. Phosphinothricin acetyl transferase
D. All of these
Question 54
Which of the following gene is transferred to plants that detoxify the herbicide atrazine?
A. Nitrilase
B. Glutathione S-transferase (GST)
C. Phosphinothrium acetyl transferase
D. All of these
Question 55
Which of the following genes can be used for making resistances against viral infection?
A. Genes for capsid protein
B. Gene for nucleocapsid protein
C. Satellite RNA
D. All of these
Question 56
Which of the following has been widely used to provide resistance against plant viruses?
A. Virus resistance genes from bacteria
B. Expression of virus coat protein genes in transgenic plants
C. Expression of anti-virus genes in vectors that transmit viruses
D. Expression of ribonuclease (RNase) genes in host plants
Question 57
Which of the following is not a characteristic of a transgenic crop?
A. herbicide resistance
B. Bt insect resistance toxin
C. increased methionine content
D. none of these
Question 58
Which of the following metabolites are implicated in stress tolerance?
A. Proline
B. Betaines
C. Both (a) and (b)
D. Citrate
Question 59
Which of the following self-pollinating plant/(s) tend to be homozygous?
A. Peas
B. Tomato
C. Peach
D. All of these