Vector Biology MCQs : This section focuses on the "Vector Biology". These Multiple Choice Questions (MCQs) should be practiced to improve the Vector Biology skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.
Question 1
Which of the following is true for a plasmid?
A. Contains an origin of replication
B. Imparts a useful characteristic to the host bacterium
C. Possesses one or more genes
D. Replicates only when the host genome is undergoing replication
Question 2
What are the possible ways through which a plasmid can replicate?
A. Using cell’s own replicative enzymes
B. Carry genes that code for special replicative enzymes
C. Replicate by inserting themselves into a bacterial chromosome
D. Carry genes that codes enzymes
Question 3
Which among the following are the smallest plasmid and an ideal cloning vector?
A. ColE1
B. RP4
C. PUC8
D. F
Question 4
What are relaxed plasmids?
A. The ones having copy numbers above 50 per cell
B. The ones that can’t be digested by endonucleases
C. The ones that can’t be isolated
D. The ones present in more than one strains of the same host
Question 5
How can conjugative and non-conjugative plasmids be differentiated?
A. On the basis of size
B. Presence of antibiotic resistance
C. Number of cloning and digestion sites
D. Presence of transfer genes
Question 6
To be able to coexist in the same cell, different plasmids must be ______
A. Conjugative
B. Of high copy number
C. Stable at high temperatures
D. Compatible
Question 7
Which of the following is not a type of plasmid?
A. F
B. R
C. Ti
D. T4
Question 8
Which characteristics do F-plasmids confer to the host bacterium?
A. Antibiotic resistance
B. Fluorescent colonies
C. Conjugative ability
D. Virulence
Question 9
Col plasmids are responsible for ______
A. Degrading unusual molecules
B. Taking up colchicine stain
C. Coding for colicins
D. None of the above
Question 10
Ti plasmids are ____ plasmids.
A. Tumor inducing
B. Degradation
C. High copy number
D. Mammalian
Question 11
Protection by covalent attachment of proteins in a plasmid, is a method for?
A. increasing the conjugative abilities
B. protection of linear DNA
C. increasing the copy number
D. increasing cloning efficiency
Question 12
Which of the following is not true for a bacteriophage?
A. A very simple structure
B. Consist either DNA or RNA
C. Bacteriophages are viruses
D. Complex structure that infects bacteria
Question 13
What is the capsid (protective coat) of the bacteriophage made up of?
A. DNA
B. RNA
C. Protein
D. Organic acids
Question 14
Which of the following is an example of head-and-tail bacteriophage?
A. M13
B. Lambda phage
C. Pbr322
D. M16
Question 15
The cycle which is completed quickly in the infection by a phage is ________
A. Lysogenic
B. Lytic
C. Replication
D. Capsid formation
Question 16
Which is a reason of instability of phage DNA molecule in the host cell in a lytic cycle?
A. The huge size of phage DNA
B. Inability of replicative enzymes
C. Immediate synthesis of capsid
D. Lytic cycle inefficiency
Question 17
Which of the following statements is not true in the context of infection by an M13 phage?
A. Lytic phage
B. Lysogenic phage
C. New phage particles are continually synthesized
D. The DNA is not integrated in host genome
Question 18
Approximate size of lambda phage is _______
A. 23 kb
B. 100 kb
C. 49 kb
D. 12 kb
Question 19
What are sticky ends?
A. Ends of M13 vector
B. 12 nucleotide stretch in lambda phage
C. The replicated product phage DNA
D. Ends on two sides of origin