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Vectors Uses for Animal Cell Culture MCQ Questions & Answers

Vectors Uses for Animal Cell Culture MCQs : This section focuses on the "Vectors Uses for Animal Cell Culture". These Multiple Choice Questions (MCQs) should be practiced to improve the Vectors Uses for Animal Cell Culture skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations.




Question 1

Polyhedrin protein is

A. non essential for baculo virus replication
B. major component of protein matrix in which viruses are embedded
C. both (a) and (b)
D. essential for baculo virus replication

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Question 2

Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

A. transcriptional regulators and are essential for viral replication
B. not necessary for viral replication
C. coding for virus coat protein
D. important for packaging of viral DNA into viral head

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Question 3

Maximum amount of foreign DNA introduced into SV 40 viruses is

A. 10 kb
B. 5 kb
C. 2.5 kb
D. 1 kb

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Question 4

Which of the following viruses are used as vectors for transferring genes to animal cells?

A. SV 40 virus
B. Bovine papilloma virus (BVP)
C. Retroviruses
D. All of the above

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Question 5

Yeast Replicating plasmids (YRp) carry

A. centromere
B. telomere
C. autonomously replicating sequence
D. both (a) and (c)

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Question 6

SV 40 vectors, in which early region replacements are carried out, require

A. COS cell line
B. SV 40 helper virus
C. pBR 322 vector
D. None of the above

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Question 7

Which of the following viruses are used as vectors for transferring genes to animal cells?

A. SV 40 virus
B. Bovine papilloma virus (BVP)
C. Retroviruses
D. All of these

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Question 8

Autonomously replicating sequence is not present in

A. YIp
B. YRp
C. YCp
D. YAC

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Question 9

Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

A. Contains centromeric sequences
B. Segregates during meiosis and mitosis
C. Functions as true chromosome
D. All of the above

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Question 10

Cloning genes in the yeast offer which of following advantage(s)?

A. Over production of proteins of commercial value
B. Ability to glycosylate proteins during secretion
C. Ability to clone large piece of DNA
D. All of the above

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Question 11

Which of the following is/are commonly used promoter of S. cerevisiae?

A. Galactokinase (GAL 1)
B. Alcohol dehydrogenase II (ADH 2)
C. Phosphoglycerate kinase (PGK)
D. All of the above

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Question 12

Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

A. Vaccinia virus
B. Baculo virus
C. Papilloma virus
D. Retrovirus

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Question 13

α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

A. His 3
B. Lys 2
C. Ura 3
D. Trp 1

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Question 14

SV 40 vectors, in which early region replacements are carried out, require

A. COS cell line
B. SV 40 helper virus
C. pBR 322 vector
D. none of these

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Question 15

Which of the following can be used for reducing the size of gene inserts containing introns?

A. Vaccinia virus
B. Baculo virus
C. Retro virus
D. none of these

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Question 16

Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

A. episomally only
B. integrated only
C. either episomally or integrated at high copy number
D. either episomally or integrated at low copy number

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Question 17

In case of Baculoviruses, the foreign gene

A. is inserted downstream of polyhedrin promoter
B. is inserted downstream of viral origin of replication
C. is inserted between the polyhedrin promoter and downstream sequences
D. is inserted randomly in viral genome

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Question 18

Which of the following(s) is/are the most commonly used markers in yeast?

A. His 3
B. Ura 3
C. Trp 1
D. All of these

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Question 19

Polyhedrin protein is

A. non essential for baculo virus replication
B. major component of protein matrix in which viruses are embedded
C. Both A and B
D. essential for baculo virus replication

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Question 20

Maximum insert size in case of adenovirus vectors is

A. 6-8 kb
B. 4-6 kb
C. 10-12 kb
D. 20 kb

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Question 21

Retroviruses consist of

A. Ds DNA genome
B. Ss DNA genome
C. RNA genome
D. cDNA genome

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Question 22

Which one of the following is the most stable vector?

A. YEp
B. YRp
C. YAC
D. YIp

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Question 23

Which of the following are produced in high copy number?

A. YEp
B. YRp
C. Both (a) and (b)
D. YCp

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Question 24

Cos cell line is

A. cohesive end site of phage λ
B. derivative of permissive CV-1 monkey cell line
C. cohesive initial site of phage λ
D. derivative of non-permissive CV-1 monkey cell line

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Question 25

Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

A. Allows transformation by using crossing over
B. Has a origin of replication and rep genes
C. Carries autonomously replicating sequence
D. Carries centromere and telomere sequences

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Question 26

Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

A. Allows transformation by using crossing over
B. Has a origin of replication and rep genes
C. Carries autonomously replicating sequence
D. Carries centromere and telomere sequences

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Question 27

YAC vectors can be used to obtain artificial chromosomes because it carries

A. centromere
B. telomere
C. autonomously replicating sequence
D. all of these

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Question 28

5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

A. His 3
B. Lys 2
C. Ura 3
D. Trp 1

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Question 29

SV 40 vectors, in which late region replacements are done, require

A. COS cell line
B. SV 40 helper virus
C. pBR 322 vector
D. none of these

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Question 30

The size of the genome of SV 40 is

A. 7.2 kb
B. 69 kb
C. 5.2 kb
D. 110 kb

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Question 31

Recombinant SV40 viruses introduce the foreign DNA into cells

A. with the DNA mediated transfection
B. without the DNA mediated transfection
C. with the RNA mediated transfection
D. without the RNA mediated transfection

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Question 32

Which of the following promoters is repressed in presence of glucose?

A. Galactokinase (GAL 1)
B. Acid phosphatase (PHOS)
C. Both (a) and (b)
D. Phosphoglycerate kinase (PGK)

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Question 33

Lytic infection of SV 40 does not occur in

A. permissive cells
B. non permissive cells
C. both (a) and (b)
D. none of these

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Question 34

Which of the following histone is not present in SV40 virus?

A. H1
B. H2A
C. H3
D. H4

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Question 35

Yeast Replicating plasmids (YRp) carry

A. centromere
B. telomere
C. autonomously replicating sequence
D. Both A and C

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Question 36

Moloney-Mu-leukemia virus is an example of

A. Vaccinia virus
B. Baculo virus
C. Retro virus
D. none of these

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Question 37

Retroviruses have advantage for being used as vector for animal cells because

A. they cover a wide host range
B. infection does not lead to cell death, infected cells produce virus over an indefinite period
C. viral gene expression is driven by strong promoters
D. All of the above

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Question 38

Bovine papilloma virus has a circular double stranded DNA genome of about

A. 2.5 kb
B. 5.2 kb
C. 7.9 kb
D. 9.5 kb

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Question 39

Yeast episomal plasmids (YEp) were constructed using

A. 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
B. 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
C. 12μm yeast plasmid and E coli vector pMB 9
D. 12μm yeast plasmid and E coli vector pBR 322

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